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MARS Antibody (PA5-115547) in ICC/IF
Immunocytochemistry analysis of MARS in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115547). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115547
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MARS(Accession P56192), corresponding to amino acid residues G570-Y620.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900183
Product Specific Information
Antibody detects endogenous levels of total MARS.
Target Information
Methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction:ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytosolic methionyl-tRNA synthetase; methionine tRNA ligase; Methionine--tRNA ligase, cytoplasmic; methionine-tRNA synthetase; methionyl trna synthetase; Methionyl-tRNA synthetase; methionyl-tRNA synthetase, cytoplasmic; MetRS
Gene Aliases: CMT2U; ILFS2; ILLD; MARS; MARS1; METRS; MRS; MTRNS; SPG70
UniProt ID: (Human) P56192, (Mouse) Q68FL6
Entrez Gene ID: (Human) 4141, (Rat) 299851, (Mouse) 216443
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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