|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues at the carboxyl terminus of human MAVS|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
It is not recommended to aliquot this antibody.
Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB, or TBK1 and IKKE (IKBKE; MIM 605048), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Viral suppressors of the RIG-I-mediated interferon response are pre-packaged in influenza virions.
PA5-17256 was used in immunocytochemistry to use three-dimensional stochastic optical reconstruction microscopy to visualize incoming influenza A virus
|Liedmann S,Hrincius ER,Guy C,Anhlan D,Dierkes R,Carter R,Wu G,Staeheli P,Green DR,Wolff T,McCullers JA,Ludwig S,Ehrhardt C||Nature communications (5:null)||2014|