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Invitrogen
MAX Polyclonal Antibody
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FIGURE: 1 / 7
MAX Antibody (PA5-79637) in ICC/IF
Immunofluorescence analysis of MAX was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MAX Polyclonal Antibody (Product # PA5-79637) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 f... View More
Product Details
PA5-79637
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MAX recombinant protein (Position: A30-R106).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEL whole cell, human PC-3 whole cell, mouse lung tissue. ICC/IF: A549 cell. Flow: THP-1 cell.
Target Information
The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: bHLHd4; Class D basic helix-loop-helix protein 4; Delta-Max; MAX; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; Myc-associated factor X; Myc-binding novel HLH/LZ protein; orf1; Protein max; Protein myn; unnamed protein product
Gene Aliases: AA960152; AI875693; BHLHD4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; MAX; Myn; PDMCS
UniProt ID: (Human) P61244, (Mouse) P28574
Entrez Gene ID: (Human) 4149, (Mouse) 17187
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
protein dimerization activity
E-box binding
DNA-binding transcription factor binding
sequence-specific double-stranded DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
protein complex binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription from RNA polymerase II promoter
protein complex assembly
negative regulation of gene expression
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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