|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides RQVGPQSGEVKEAPR and PEEDKEENKDDSASK, corresponding to amino acids 98-113 and 342-356 of Human MBD1, respectively.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29797 detects MBD1 from human samples.
PA1-29797 has been successfully used in Western blot applications.
The PA1-29797 immunogen is: Synthetic peptides RQVGPQSGEVKEAPR and PEEDKEENKDDSASK, corresponding to amino acids 98-113 and 342-356 of Human MBD1, respectively.
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The
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