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Invitrogen
MBD2 Polyclonal Antibody
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FIGURE: 1 / 2
MBD2 Antibody (PA5-115551) in ICC/IF
Immunocytochemistry analysis of MBD2 in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115551). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Product Details
PA5-115551
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MBD2(Accession Q9UBB5), corresponding to amino acid residues F206-V256.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total MBD2.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Demethylase; DKFZp586O0821; DMTase; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2
Gene Aliases: DMTase; MBD2; MBD2a; NY-CO-41
UniProt ID: (Human) Q9UBB5, (Mouse) Q9Z2E1
Entrez Gene ID: (Human) 8932, (Mouse) 17191
DNA binding
chromatin binding
satellite DNA binding
mRNA binding
protein binding
methyl-CpG binding
protein domain specific binding
nucleosomal DNA binding
siRNA binding
identical protein binding
binding, bridging
C2H2 zinc finger domain binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
ventricular cardiac muscle tissue development
chromatin remodeling
methylation-dependent chromatin silencing
regulation of transcription from RNA polymerase II promoter
response to mechanical stimulus
Wnt signaling pathway
positive regulation of Wnt signaling pathway
response to nutrient levels
response to estradiol
regulation of gene expression, epigenetic
regulation of cell proliferation
maternal behavior
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
embryonic organ development
protein-containing complex assembly
response to bisphenol A
cellular response to serotonin
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of chromatin binding
ATP-dependent chromatin remodeling
cellular protein complex assembly
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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