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FIGURE: 1 / 1
MBD3 Antibody (PA5-120806) in WB
Western blot analysis of MBD3 in extracts of various cell lines. Samples were incubated with MBD3 Polyclonal antibody (Product # PA5-120806) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120806
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human MBD3 (NP_001268383.1).
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914378
Product Specific Information
Positive test controls include: Jurkat, K-562. The target is usually found in the following locations: Chromosome, Nucleus.^M
^M
Immunogen sequence: MERKSPSGKK FRSKPQLARY LGGSMDLSTF DFRTGKMLMS KMNKSRQRVR YDSSNQVKGK PDLNTALPVR QTASIFKQPV TKITNHPSNK VKSDPQKAVD QPRQLFWEKK LSGLNAFDIA EELVKTMDLP KGLQGVGPGC TDETLLSAIA SALHTSTMPI TGQLSAAVEK NPGVWLNTTQ PLCKAFMVTD EDIRKQEELV QQVRKRLEEA LMADMLAHVE ELARDGEAPL DKACAEDDDE EDEEEEEEEP DPDPE
Target Information
Regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. Necessary for centrosome duplication. It functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CpG binding domain 3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
Gene Aliases: AI181826; AU019209; MBD3
UniProt ID: (Human) O95983, (Mouse) Q9Z2D8
Entrez Gene ID: (Human) 53615, (Mouse) 17192
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
methylation-dependent chromatin silencing
transcription, DNA-templated
brain development
heart development
aging
tissue development
histone acetylation
histone deacetylation
response to nutrient levels
response to estradiol
ATP-dependent chromatin remodeling
regulation of DNA methylation
embryonic organ development
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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