Invitrogen

MC1R Polyclonal Antibody

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MC1R Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 3 Immunofluorescence staining of MC1R and Nurr1 with microglia in the brain at 48 h post-HI. Immunofluorescence staining showed an increase expression of MC1R ( a ) and Nurr1 ( b ) on microglia in the vehicle group when compared with the sham group, and further increased expression of MC1R ( a ) and Nurr1 ( b ) in the BMS-470359 treatment group. Merge showed the colocalization of MC1R and Nurr1 on microglia. Microglia were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. n = 2 per group. Scale bar = 100 mum

MC1R Antibody in Immunohistochemistry (IHC) — Figure 3 Immunofluorescence staining showed MC1R and Nurr1 colocalization with neurons in the ipsilateral brain hemisphere at 48 h post-HI. Immunofluorescence staining showed that MC1R (a) and Nurr1 (b) expressions on neurons were seen to be higher in the vehicle-treated pup rats compared to the sham group, and a higher expression of MC1R (a) and Nurr1 (b) on neurons after BMS-470359 treatment compared with vehicle. Merge showed the colocalization of MC1R and Nurr1 on neurons. n = 2 per group. Neurons were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. Scale bar = 100 mu m.

MC1R Antibody in Western Blot (WB) — Figure 4 Effects of BMS-470539 treatment on neuronal apoptosis at 48 h post-HI, and the knockout efficiency of MC1R or Nurr1 knockout CRISPR in naive and HI rats. (a, b) Representative microphotographs and quantitative analysis of C-Cas 3-positive neurons in the ipsilateral hemisphere at 48 h post-HI. The number of C-Cas 3-positive neurons significantly increased in the vehicle group compared with the sham group and BMS-470539 treatment group. n = 4 per group. C-Cas 3 was green. Neurons were red. Blue was for DAPI. Scale bar = 100 mu m. (c, d) Representative Western blot bands and quantitative analysis of MC1R and Nurr1 protein levels in the ipsilateral hemisphere at 48 h post-HI. The expression of MC1R or Nurr1 was significantly reduced by MC1R or Nurr1 knockout CRISPR in naive and HI rats. n = 4 per group (data were represented as mean +- SD; * p < 0.05 versus sham; # p <0.05 versus HI+vehicle; @ p < 0.05 versus naive+control CRISPR, & p < 0.05 versus HI+control CRISPR; one-way ANOVA, Tukey's post hoc test).

Product Details

PA5-75342

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 300-350 of Human MC1R.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2719070

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

MC1R, a Melanocortin Receptor, mediates the effects of melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). MSH and its receptor, MC1R, are key regulators of melanogenesis. They control the relative proportions of red pheomelanin and black photoprotective eumelanin in the skin by increasing the production of eumelanin. Non-functional, truncated forms of the receptor lead to lighter coat color in animals. In contrast, constitutively active receptors lead to darker color.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Fri Mar 18 00:00:00 EDT 2022

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

PA5-75342 was used in Immunohistochemistry, Western Blot to study the neuroprotective mechanisms of the BMS-470539/MC1R system which attunates early brain injury following subarachnoid hemorrhage by suppressing the oxidative stress, apoptosis, and mitochondrial fission through the AMPK/SIRT1/PGC-1α signaling pathway.

Wed Aug 04 00:00:00 EDT 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Wed Aug 04 00:00:00 EDT 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Wed Aug 04 00:00:00 EDT 2021

Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect.

Theranostics

Fan K,Zeng L,Guo J,Xie S,Yu Y,Chen J,Cao J,Xiang Q,Zhang S,Luo Y,Deng Q,Zhou Q,Zhao Y,Hao L,Wang Z,Zhong L

PA5-75342 was used in Immunohistochemistry to provide a promising strategy of Dex/PFP@LIPs-BMS-α as effective and safe against immune-associated nephropathy.

Tue Jul 27 00:00:00 EDT 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Tue Jan 19 00:00:00 EST 2021

Activation of Melanocortin 1 Receptor Attenuates Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage viathe Suppression of Neuroinflammation through AMPK/TBK1/NF-κB Pathway in Rats.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Xu W,Mo J,Ocak U,Travis ZD,Enkhjargal B,Zhang T,Wu P,Peng J,Li T,Zuo Y,Shao A,Tang J,Zhang J,Zhang JH

PA5-75342 was used in Immunohistochemistry (PFA fixed) to study the hypothesis that activation of melanocortin 1 receptor (MC1R) with BMS-470539 attenuates EBI by suppression of neuroinflammation after SAH.

Wed Jan 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: alpha melanocyte stimulating hormone receptor; extension recessive yellow; MC1 receptor; MC1-R; melanocortin 1 receptor; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; melanocortin-1 receptor; Melanocyte-stimulating hormone receptor; melanotropin receptor; MSH-R; tobacco darkening

Gene Aliases: CMM5; e; MC1R; MSH-R; MSHR; Mshra; SHEP2; Tob

UniProt ID: (Human) Q01726, (Mouse) Q01727

Entrez Gene ID: (Human) 4157, (Rat) 102552838, (Mouse) 17199

Molecular Function: G-protein coupled receptor