Invitrogen

MC1R Polyclonal Antibody

11 Published Figures

View all (17) MC1R antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

FIGURE: 1 / 12

MC1R Antibody (PA5-75342) in WB

Western blot analysis of MC1R in various lysates. Samples were incubated with MC1R polyclonal antibody (Product # PA5-75342) using a dilution of 1:1,000. Lane 1: Hela whole cell lysate (40 µg); Lane 2: The Brain tissue lysate of Rat (40 µg); Lane 3: The Brain tissue lysate of Mouse (40 µg).

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Immunohistochemistry (Frozen) (IHC (F))

MC1R Antibody in Immunohistochemistry (IHC)

MC1R Antibody in Immunohistochemistry (IHC)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

MC1R Antibody in Western Blot (WB)

Product Image Gallery

MC1R Antibody in Western Blot (WB) — Western blot analysis of MC1R in various lysates. Samples were incubated with MC1R polyclonal antibody (Product # PA5-75342) using a dilution of 1:1,000. Lane 1: Hela whole cell lysate (40 µg); Lane 2: The Brain tissue lysate of Rat (40 µg); Lane 3: The Brain tissue lysate of Mouse (40 µg).

MC1R Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 3 Immunofluorescence staining of MC1R and Nurr1 with microglia in the brain at 48 h post-HI. Immunofluorescence staining showed an increase expression of MC1R ( a ) and Nurr1 ( b ) on microglia in the vehicle group when compared with the sham group, and further increased expression of MC1R ( a ) and Nurr1 ( b ) in the BMS-470359 treatment group. Merge showed the colocalization of MC1R and Nurr1 on microglia. Microglia were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. n = 2 per group. Scale bar = 100 mum

MC1R Antibody in Immunohistochemistry (IHC) — Figure 3 Immunofluorescence staining showed MC1R and Nurr1 colocalization with neurons in the ipsilateral brain hemisphere at 48 h post-HI. Immunofluorescence staining showed that MC1R (a) and Nurr1 (b) expressions on neurons were seen to be higher in the vehicle-treated pup rats compared to the sham group, and a higher expression of MC1R (a) and Nurr1 (b) on neurons after BMS-470359 treatment compared with vehicle. Merge showed the colocalization of MC1R and Nurr1 on neurons. n = 2 per group. Neurons were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. Scale bar = 100 mu m.

MC1R Antibody in Immunohistochemistry (IHC) — Figure 1 The expression of MC1R, SIRT1 and PGC-1alpha. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1alpha of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means +- SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 mum.

MC1R Antibody in Western Blot (WB) — Figure 4 Effects of BMS-470539 treatment on neuronal apoptosis at 48 h post-HI, and the knockout efficiency of MC1R or Nurr1 knockout CRISPR in naive and HI rats. (a, b) Representative microphotographs and quantitative analysis of C-Cas 3-positive neurons in the ipsilateral hemisphere at 48 h post-HI. The number of C-Cas 3-positive neurons significantly increased in the vehicle group compared with the sham group and BMS-470539 treatment group. n = 4 per group. C-Cas 3 was green. Neurons were red. Blue was for DAPI. Scale bar = 100 mu m. (c, d) Representative Western blot bands and quantitative analysis of MC1R and Nurr1 protein levels in the ipsilateral hemisphere at 48 h post-HI. The expression of MC1R or Nurr1 was significantly reduced by MC1R or Nurr1 knockout CRISPR in naive and HI rats. n = 4 per group (data were represented as mean +- SD; * p < 0.05 versus sham; # p <0.05 versus HI+vehicle; @ p < 0.05 versus naive+control CRISPR, & p < 0.05 versus HI+control CRISPR; one-way ANOVA, Tukey's post hoc test).

MC1R Antibody in Western Blot (WB) — Figure 5 BMS-470539 treatment exerted its antioxidative stress and antiapoptosis effects via MC1R/cAMP/PKA/Nurr1 signaling pathway at 48 h post-HI. (a) Representative Western blot bands. (b-i) Quantification of MC1R, cAMP, p-PKA, Nurr1, 4-HNE, HO-1, Bax, and Bcl-2 expression levels in the ipsilateral hemisphere at 48 h post-HI. BMS-470539 treatment significantly increased the protein levels of MC1R, cAMP, p-PKA, Nurr1, HO-1, and Bcl-2 but significantly decreased the expression of 4-HNE and Bax compared to the HI+vehicle group. Knockout of MC1R or Nurr1 with CRISPR interventions significantly abolished such effects of BMS-470539. n = 6 per group. Data was represented as mean +- SD. * p < 0.05 versus sham, # p < 0.05 versus HI+vehicle, @ p < 0.05 HI+BMS-470539 or HI+BMS-470539+control CRISPR; one-way ANOVA, Tukey's post hoc test.

MC1R Antibody in Western Blot (WB) — Figure 1 Temporal expression of endogenous MC1R and Nurr1 in the ipsilateral brain hemisphere post-HI. (a) Representative Western blot bands of the time course. (b) Western blot data showed that the endogenous expression levels of MC1R significantly increased from 12 h reaching peak at 48 h post-HI. (c) Nurr1 expression levels significantly increased over time, reached highest at the 48 h post-HI. n = 6 per group. Data were represented as mean +- SD. * p < 0.05 versus sham, # p < 0.05 versus 6 h HI, and & p < 0.05 versus 48 h HI; one-way ANOVA, Tukey's post hoc test.

MC1R Antibody in Western Blot (WB) — Fig. 8 MC1R activation on neuroinflammation via cAMP/PKA/Nurr1 signaling pathway at 48 h post-HI. a Representative picture of Western blot data showing bands of the expression levels of MC1R, cAMP, p-PKA, Nurr1, IL-1beta, TNFalpha, and IL-6 either with BMS-470539 treatment alone, BMS-470539 + MC1R KO CRISPR, BMS-470539 + Nurr1 KO CRISPR, and BMS-470539 + control CRISPR groups. b - h Western blot data quantification of bands showed that BMS-470539 treatment significantly increased the expression of MC1R, cAMP, p-PKA, and Nurr1 compared to the HI + vehicle group. Knockout of MC1R using CRISPR significantly decreased MC1R, cAMP, p-PKA, and Nurr1 expression levels compared to the HI + BMS-470539 group or HI + BMS-470539 + control CRISPR group. Furthermore, knockout of Nurr1 using CRISPR significantly decreased the levels of Nurr1, but did not affect MC1R, cAMP, and p-PKA expression compared to the corresponding controls. Activation of MC1R with BMS-470539 showed significantly decreased levels of IL-1beta, TNFalpha, and IL-6, while both treatment groups with CRISPR interventions significantly reversed these effects. Data was represented as mean +- SD. The one-way ANOVA was followed by Tukey''s post hoc test (* p < 0.05 versus sham; # p < 0.05 versus HI + vehicle; @ p < 0.05 HI + BMS-470539 or HI + BMS-470539 + control CRISPR; n = 6 per group)

MC1R Antibody in Western Blot (WB) — Fig. 1 Expression levels of alpha-MSH, MC1R, and Nurr1 post-HI. a Representative pictures of Western blot data. b Western blot data showed that the expression levels of alpha-MSH significantly increased at 12 h post-HI, and peaked at 24 h. c , d The endogenous expression levels of MC1R and Nurr1 increased over time and peaked at 48 h post-HI. Data were represented as mean +- SD. Statistical differences between groups were analyzed using one-way ANOVA, followed by Tukey''s post hoc test (* p < 0.05 versus sham, # p < 0.05 versus 6 h HI, @ p < 0.05 versus 24 h HI, & p < 0.05 versus 48 h HI; n = 4 per group). e Representative microphotographs of double immunofluorescence of alpha-MSH (green) with microglia (Iba-1, red), astrocyte (GFAP, red), and neuron (NeuN, red) in the peri-infarcted area at 24 h post-HI. DAPI was stained blue. Merged images showed that alpha-MSH was colocalized with microglia, astrocytes, and neurons. n = 2 per group. Scale bar = 100 mum

MC1R Antibody in Western Blot (WB) — Figure 5 Inhibition of MC1R with MSG-606 abolished the neuroprotective effects of BMS-470539 at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1alpha, UCP2, GPx, SOD, CC-3, Bcl-2, Bax; (C) ROS and ATP levels at 24 h after SAH. n = 6 for each group. Data were shown as means +- SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

MC1R Antibody in Western Blot (WB) — Figure 6 SIRT1 inhibitor, selisistat reversed the anti-oxidative stress and anti-apoptotic effects of MC1R at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1alpha, UCP2, GPx, SOD, CC-3, Bcl-2, Bax. Data were shown as means +- SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. *p < 0.05 vs. sham, &p < 0.05 vs. SAH + vehicle, #p < 0.05 vs. SAH + BMS-470539.

MC1R Antibody in Western Blot (WB) — Knockout of PGC-1alpha with siRNA aggravates oxidative stress and apoptosis following SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1alpha, UCP2, GPx, SOD, CC-3, Bcl-2, Bax. Data were shown as means +- SD, and were compared using 1-way ANOVA followed by the Tukey post hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

Product Details

PA5-75342

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 271-317 of Human MC1-R.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2719070

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

MC1R, a Melanocortin Receptor, mediates the effects of melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). MSH and its receptor, MC1R, are key regulators of melanogenesis. They control the relative proportions of red pheomelanin and black photoprotective eumelanin in the skin by increasing the production of eumelanin. Non-functional, truncated forms of the receptor lead to lighter coat color in animals. In contrast, constitutively active receptors lead to darker color.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Fri Mar 18 00:00:00 UTC 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Fri Mar 18 00:00:00 UTC 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Fri Mar 18 00:00:00 UTC 2022

BMS-470539 Attenuates Oxidative Stress and Neuronal Apoptosis via MC1R/cAMP/PKA/Nurr1 Signaling Pathway in a Neonatal Hypoxic-Ischemic Rat Model.

Oxidative medicine and cellular longevity

Yu S,Doycheva DM,Gamdzyk M,Gao Y,Guo Y,Travis ZD,Tang J,Chen WX,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Fri Mar 18 00:00:00 UTC 2022

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Tue Jan 19 00:00:00 UTC 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Wed Aug 04 00:00:00 UTC 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Wed Aug 04 00:00:00 UTC 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Western Blot

Wed Aug 04 00:00:00 UTC 2021

Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect.

Theranostics

Fan K,Zeng L,Guo J,Xie S,Yu Y,Chen J,Cao J,Xiang Q,Zhang S,Luo Y,Deng Q,Zhou Q,Zhao Y,Hao L,Wang Z,Zhong L

PA5-75342 was used in Immunohistochemistry to provide a promising strategy of Dex/PFP@LIPs-BMS-α as effective and safe against immune-associated nephropathy.

Tue Jul 27 00:00:00 UTC 2021

Activation of Melanocortin 1 Receptor Attenuates Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage viathe Suppression of Neuroinflammation through AMPK/TBK1/NF-κB Pathway in Rats.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Xu W,Mo J,Ocak U,Travis ZD,Enkhjargal B,Zhang T,Wu P,Peng J,Li T,Zuo Y,Shao A,Tang J,Zhang J,Zhang JH

PA5-75342 was used in Immunohistochemistry (PFA fixed) to study the hypothesis that activation of melanocortin 1 receptor (MC1R) with BMS-470539 attenuates EBI by suppression of neuroinflammation after SAH.

Wed Jan 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: alpha melanocyte stimulating hormone receptor; extension recessive yellow; MC1 receptor; MC1-R; melanocortin 1 receptor; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; melanocortin-1 receptor; Melanocyte-stimulating hormone receptor; melanotropin receptor; MSH-R; tobacco darkening

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Gene Aliases: CMM5; e; MC1R; MSH-R; MSHR; Mshra; SHEP2; Tob

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UniProt ID: (Human) Q01726, (Mouse) Q01727

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Entrez Gene ID: (Human) 4157, (Rat) 102552838, (Mouse) 17199

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melanocortin receptor activity melanocyte-stimulating hormone receptor activity protein binding G-protein coupled peptide receptor activity ubiquitin protein ligase binding hormone binding signal transducer activity G-protein coupled receptor activity G-protein coupled receptor

G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger multicellular organism development UV protection positive regulation of protein kinase A signaling sensory perception of pain positive regulation of cAMP biosynthetic process negative regulation of tumor necrosis factor production intracellular signal transduction melanin biosynthetic process pigmentation positive regulation of transcription from RNA polymerase II promoter positive regulation of protein kinase B signaling UV-damage excision repair positive regulation of protein kinase C signaling G-protein coupled receptor signaling pathway regulation of feeding behavior positive regulation of feeding behavior signal transduction

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