Invitrogen

MC1R Polyclonal Antibody

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MC1R Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Fig. 3 Immunofluorescence staining of MC1R and Nurr1 with microglia in the brain at 48 h post-HI. Immunofluorescence staining showed an increase expression of MC1R ( a ) and Nurr1 ( b ) on microglia in the vehicle group when compared with the sham group, and further increased expression of MC1R ( a ) and Nurr1 ( b ) in the BMS-470359 treatment group. Merge showed the colocalization of MC1R and Nurr1 on microglia. Microglia were stained red. MC1R and Nurr1 were stained green. DAPI was stained blue. n = 2 per group. Scale bar = 100 mum

MC1R Antibody in Immunohistochemistry (IHC) — Figure 1 The expression of MC1R, SIRT1 and PGC-1alpha. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1alpha of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means +- SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 mum.

MC1R Antibody in Western Blot (WB) — Fig. 8 MC1R activation on neuroinflammation via cAMP/PKA/Nurr1 signaling pathway at 48 h post-HI. a Representative picture of Western blot data showing bands of the expression levels of MC1R, cAMP, p-PKA, Nurr1, IL-1beta, TNFalpha, and IL-6 either with BMS-470539 treatment alone, BMS-470539 + MC1R KO CRISPR, BMS-470539 + Nurr1 KO CRISPR, and BMS-470539 + control CRISPR groups. b - h Western blot data quantification of bands showed that BMS-470539 treatment significantly increased the expression of MC1R, cAMP, p-PKA, and Nurr1 compared to the HI + vehicle group. Knockout of MC1R using CRISPR significantly decreased MC1R, cAMP, p-PKA, and Nurr1 expression levels compared to the HI + BMS-470539 group or HI + BMS-470539 + control CRISPR group. Furthermore, knockout of Nurr1 using CRISPR significantly decreased the levels of Nurr1, but did not affect MC1R, cAMP, and p-PKA expression compared to the corresponding controls. Activation of MC1R with BMS-470539 showed significantly decreased levels of IL-1beta, TNFalpha, and IL-6, while both treatment groups with CRISPR interventions significantly reversed these effects. Data was represented as mean +- SD. The one-way ANOVA was followed by Tukey''s post hoc test (* p < 0.05 versus sham; # p < 0.05 versus HI + vehicle; @ p < 0.05 HI + BMS-470539 or HI + BMS-470539 + control CRISPR; n = 6 per group)

Product Details

PA5-75342

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (PFA fixed) (IHC (PFA))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:50-1:200	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acids 300-350 of Human MC1R.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2719070

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

MC1R, a Melanocortin Receptor, mediates the effects of melanocyte-stimulating hormone (MSH) and adrenocorticotropic hormone (ACTH). MSH and its receptor, MC1R, are key regulators of melanogenesis. They control the relative proportions of red pheomelanin and black photoprotective eumelanin in the skin by increasing the production of eumelanin. Non-functional, truncated forms of the receptor lead to lighter coat color in animals. In contrast, constitutively active receptors lead to darker color.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Wed Aug 04 00:00:00 EDT 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Wed Aug 04 00:00:00 EDT 2021

Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats.

Theranostics

Xu W,Yan J,Ocak U,Lenahan C,Shao A,Tang J,Zhang J,Zhang JH

Published figure using MC1R polyclonal antibody (Product # PA5-75342) in Immunohistochemistry

Wed Aug 04 00:00:00 EDT 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

PA5-75342 was used in Western Blot, Immunocytochemistry to explore the role of MC1R activation on neuroinflammation and the potential underlying mechanisms after neonatal hypoxic-ischemic brain injury in rats.

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Tue Jan 19 00:00:00 EST 2021

Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats.

Journal of neuroinflammation

Yu S,Doycheva DM,Gamdzyk M,Yang Y,Lenahan C,Li G,Li D,Lian L,Tang J,Lu J,Zhang JH

Tue Jan 19 00:00:00 EST 2021

Activation of Melanocortin 1 Receptor Attenuates Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage viathe Suppression of Neuroinflammation through AMPK/TBK1/NF-κB Pathway in Rats.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Xu W,Mo J,Ocak U,Travis ZD,Enkhjargal B,Zhang T,Wu P,Peng J,Li T,Zuo Y,Shao A,Tang J,Zhang J,Zhang JH

PA5-75342 was used in Immunohistochemistry (PFA fixed) to study the hypothesis that activation of melanocortin 1 receptor (MC1R) with BMS-470539 attenuates EBI by suppression of neuroinflammation after SAH.

Wed Jan 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: alpha melanocyte stimulating hormone receptor; extension recessive yellow; MC1 receptor; MC1-R; melanocortin 1 receptor; melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; melanocortin-1 receptor; Melanocyte-stimulating hormone receptor; melanotropin receptor; MSH-R; tobacco darkening

Gene Aliases: CMM5; e; MC1R; MSH-R; MSHR; Mshra; SHEP2; Tob

UniProt ID: (Human) Q01726, (Mouse) Q01727

Entrez Gene ID: (Human) 4157, (Rat) 102552838, (Mouse) 17199

Molecular Function: G-protein coupled receptor