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Western blot of MCF2L in (A) Jurkat and (B) human thymus lysates was performed using 35ug protein in RIPA buffer. Lysates were probed with a MCF2L polyclonal antibody (Product# PA5-37934) at a dilution of 1ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-QLDNQATVQR, from the internal region of MCF2L|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog and cow based on sequence homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ARHGEF14; DBL's big sister; DBS; FLJ12122; guanine nucleotide exchange factor DBS; KIAA0362; MCF.2 cell line derived transforming sequence-like; MCF2 transforming sequence-like protein; OST
ARHGEF14; DBS; KIAA0362; MCF2L; OST