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MCL1 Polyclonal Antibody

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MCL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (Product # 16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 16225-1-AP ( MCL1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MCL1 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCL1 Antibody in Immunoprecipitation (IP) — IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.

Product Details

16225-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 32 publications 32 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCL1 Fusion Protein Ag10397 (1-327 aa encoded by BC107735) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.34 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MFGLKRNAV IGLNLYCGGA GLGAGSGGAT RPGGRLLATE KEASARREIG GGEAGAVIGG SAGASPPSTL TPDSRRVARP PPIGAEVPDV TATPARLLFF APTRRAAPLE EMEAPAADAI MSPEEELDGY EPEPLGKRPA VLPLLELVGE SGNNTSTDGS LPSTPPPAEE EEDELYRQSL EIISRYLREQ ATGAKDTKPM GRSGATSRKA LETLRRVGDG VQRNHETAFQ GMLRKLDIKN EDDVKSLSRV MIHVFSDGVT NWGRIVTLIS FGAFVAKHLK TINQESCIEP LAESITDVLV RTKRDWLVKQ RGWDGFVEFF HVEDLEGG (1-327 aa encoded by BC107735)

Target Information

MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; Induced myeloid leukemia cell differentiation protein Mcl-1; Induced myeloid leukemia cell differentiation protein Mcl-1 homolog; MCL-1S; mcl1/EAT; MGC104264; MGC1839; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)

Gene Aliases: AW556805; bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM

UniProt ID: (Human) Q07820, (Mouse) P97287, (Rat) Q9Z1P3

Entrez Gene ID: (Human) 4170, (Mouse) 17210, (Rat) 60430

Function(s)

cell fate determination multicellular organism development intrinsic apoptotic signaling pathway in response to DNA damage apoptotic mitochondrial changes cellular homeostasis response to cytokine negative regulation of apoptotic process protein transmembrane transport extrinsic apoptotic signaling pathway in absence of ligand positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of anoikis regulation of response to DNA damage stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway apoptotic process cell differentiation regulation of apoptotic process

Process(es)

protein binding protein channel activity protein homodimerization activity protein heterodimerization activity BH3 domain binding protein dimerization activity BH domain binding

MCL1 Polyclonal Antibody

Product Details

16225-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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