|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide of 38 amino acids representing a portion of human MCM 3 encoded by exons 10 and 11|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2-5 µg/mg of lysate|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21998 detects MCM3 in Human samples.
PA1-21998 has been successfully used in Immunoprecipitation and Western Blot procedures.
PA1-21998 immunogen corresponds to Synthetic peptide of 38 amino acids representing a portion of human MCM 3 encoded by exons 10 and 11.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.