Proteintech

MCM5 Polyclonal Antibody

Product Image Gallery

MCM5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11703-1-AP (MCM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 11703-1-AP (MCM5 antibody) at dilution of 1:100 (under 10x lens).

MCM5 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MCM5 Antibody in Immunoprecipitation (IP) — IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:500) with Jurkat cells lysate 2400ug.

Product Details

11703-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:8,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCM5 Fusion Protein Ag2271 (388-735 aa encoded by BC000142) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: SQLLKFVEK CSPIGVYTSG KGSSAAGLTA SVMRDPSSRN FIMEGGAMVL ADGGVVCIDE FDKMREDDRV AIHEAMEQQT ISIAKAGITT TLNSRCSVLA AANSVFGRWD ETKGEDNIDF MPTILSRFDM IFIVKDEHNE ERDVMLAKHV ITLHVSALTQ TQAVEGEIDL AKLKKFIAYC RVKCGPRLSA EAAEKLKNRY IIMRSGARQH ERDSDRRSSI PITVRQLEAI VRIAEALSKM KLQPFATEAD VEEALRLFQV STLDAALSGT LSGVEGFTSQ EDQEMLSRIE KQLKRRFAIG SQVSEHSIIK DFTKQKYPEH AIHKVLQLML RRGEIQHRMQ RKVLYRLK (388-735 aa encoded by BC000142)

Target Information

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MCMs expression in lung cancer: implication of prognostic significance.

Journal of Cancer

Liu YZ,Wang BS,Jiang YY,Cao J,Hao JJ,Zhang Y,Xu X,Cai Y,Wang MR

Fri Apr 08 00:00:00 EDT 2022

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Cancer cytopathology

Liu YZ,Jiang YY,Wang BS,Hao JJ,Shang L,Zhang TT,Cao J,Xu X,Zhan QM,Wang MR

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance deficient 5 (cell division cycle 46); minichromosome maintenance deficient 5, cell division cycle 46; P1-CDC46

Gene Aliases: AA617332; AI324988; AL033333; CDC46; MCM5; Mcmd5; P1-CDC46

UniProt ID: (Human) P33992, (Mouse) P49718

Entrez Gene ID: (Human) 4174, (Rat) 291885, (Mouse) 17218

Molecular Function: DNA metabolism protein