Proteintech

MCM5 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (9)

FIGURE: 1 / 9

MCM5 Antibody (11703-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 11703-1-AP) MCM5 antibody at dilution of 1:8,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

MCM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 11703-1-AP (MCM5 antibody) at dilution of 1:100 (under 10x lens).

MCM5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11703-1-AP (MCM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCM5 Antibody in Immunoprecipitation (IP) — IP result of anti-MCM5 (Product # 11703-1-AP), 4 µg; Detection (Product # 11703-1-AP) 1:4,000 with Jurkat cells lysate 2400 µg.

Product Details

11703-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:8,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MCM5 Fusion Protein Ag2271 (388-735 aa encoded by BC000142)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: SQLLKFVEK CSPIGVYTSG KGSSAAGLTA SVMRDPSSRN FIMEGGAMVL ADGGVVCIDE FDKMREDDRV AIHEAMEQQT ISIAKAGITT TLNSRCSVLA AANSVFGRWD ETKGEDNIDF MPTILSRFDM IFIVKDEHNE ERDVMLAKHV ITLHVSALTQ TQAVEGEIDL AKLKKFIAYC RVKCGPRLSA EAAEKLKNRY IIMRSGARQH ERDSDRRSSI PITVRQLEAI VRIAEALSKM KLQPFATEAD VEEALRLFQV STLDAALSGT LSGVEGFTSQ EDQEMLSRIE KQLKRRFAIG SQVSEHSIIK DFTKQKYPEH AIHKVLQLML RRGEIQHRMQ RKVLYRLK (388-735 aa encoded by BC000142)

Target Information

The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N⁶-methyladenosine-dependent manner.

Signal transduction and targeted therapy

Yang Y,Yan Y,Yin J,Tang N,Wang K,Huang L,Hu J,Feng Z,Gao Q,Huang A

Fri Feb 10 00:00:00 EST 2023

O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N⁶-methyladenosine-dependent manner.

Signal transduction and targeted therapy

Yang Y,Yan Y,Yin J,Tang N,Wang K,Huang L,Hu J,Feng Z,Gao Q,Huang A

Fri Feb 10 00:00:00 EST 2023

O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N⁶-methyladenosine-dependent manner.

Signal transduction and targeted therapy

Yang Y,Yan Y,Yin J,Tang N,Wang K,Huang L,Hu J,Feng Z,Gao Q,Huang A

Fri Feb 10 00:00:00 EST 2023

O-GlcNAcylation of YTHDF2 promotes HBV-related hepatocellular carcinoma progression in an N⁶-methyladenosine-dependent manner.

Signal transduction and targeted therapy

Yang Y,Yan Y,Yin J,Tang N,Wang K,Huang L,Hu J,Feng Z,Gao Q,Huang A

Fri Feb 10 00:00:00 EST 2023

The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang Y,Wang H,Zhu Y,Hou X,Li X,Zhou X,Ge L,Xu J,Su Y

Mon May 01 00:00:00 EDT 2023

The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang Y,Wang H,Zhu Y,Hou X,Li X,Zhou X,Ge L,Xu J,Su Y

Mon May 01 00:00:00 EDT 2023

m⁶ A-Dependent Modulation via IGF2BP3/MCM5/Notch Axis Promotes Partial EMT and LUAD Metastasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yang X,Bai Q,Chen W,Liang J,Wang F,Gu W,Liu L,Li Q,Chen Z,Zhou A,Long J,Tian H,Wu J,Ding X,Zhou N,Li M,Yang Y,Cai J

Sat Jul 01 00:00:00 EDT 2023

m⁶ A-Dependent Modulation via IGF2BP3/MCM5/Notch Axis Promotes Partial EMT and LUAD Metastasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Yang X,Bai Q,Chen W,Liang J,Wang F,Gu W,Liu L,Li Q,Chen Z,Zhou A,Long J,Tian H,Wu J,Ding X,Zhou N,Li M,Yang Y,Cai J

Sat Jul 01 00:00:00 EDT 2023

ITRAQ-based quantitative proteomic analysis reveals that VPS35 promotes the expression of MCM2-7 genes in HeLa cells.

Scientific reports

Hong X,Wang T,Du J,Hong Y,Yang CP,Xiao W,Li Y,Wang M,Sun H,Deng ZH

Sat Jun 11 00:00:00 EDT 2022

The Association of R-Loop Binding Proteins Subtypes with CIN Implicates Therapeutic Strategies in Colorectal Cancer.

Cancers

Zhao W,Pei Q,Zhu Y,Zhan D,Mao G,Wang M,Qiu Y,Zuo K,Pei H,Sun LQ,Wen M,Tan R

Tue Nov 15 00:00:00 EST 2022

Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.

Journal of nanobiotechnology

Zhao N,Gui X,Fang Q,Zhang R,Zhu W,Zhang H,Li Q,Zhou Y,Zhao J,Cui X,Gao G,Tang H,Shen N,Chen T,Song H,Shen W

Sat Apr 02 00:00:00 EDT 2022

lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.

Molecular therapy : the journal of the American Society of Gene Therapy

Jiang Y,Guo H,Tong T,Xie F,Qin X,Wang X,Chen W,Zhang J

Wed Jan 05 00:00:00 EST 2022

lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.

Molecular therapy : the journal of the American Society of Gene Therapy

Jiang Y,Guo H,Tong T,Xie F,Qin X,Wang X,Chen W,Zhang J

Wed Jan 05 00:00:00 EST 2022

lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5.

Molecular therapy : the journal of the American Society of Gene Therapy

Jiang Y,Guo H,Tong T,Xie F,Qin X,Wang X,Chen W,Zhang J

Wed Jan 05 00:00:00 EST 2022

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

eLife

Saito Y,Santosa V,Ishiguro KI,Kanemaki MT

Tue Apr 19 00:00:00 EDT 2022

WASHC1 interacts with MCM2-7 complex to promote cell survival under replication stress.

Molecular biology reports

Hong Y,Sun H,Hong X,Yang CP,Billadeau DD,Wang T,Deng ZH

Thu Sep 01 00:00:00 EDT 2022

WASHC1 interacts with MCM2-7 complex to promote cell survival under replication stress.

Molecular biology reports

Hong Y,Sun H,Hong X,Yang CP,Billadeau DD,Wang T,Deng ZH

Thu Sep 01 00:00:00 EDT 2022

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Ecotoxicology and environmental safety

Li Z,Yin H,Shen Y,Ren M,Xu X

Mon Mar 15 00:00:00 EDT 2021

LncRNA CARMN overexpression promotes prognosis and chemosensitivity of triple negative breast cancer via acting as miR143-3p host gene and inhibiting DNA replication.

Journal of experimental & clinical cancer research : CR

Sheng X,Dai H,Du Y,Peng J,Sha R,Yang F,Zhou L,Lin Y,Xu S,Wu Y,Yin W,Lu J

Wed Jun 23 00:00:00 EDT 2021

LncRNA CARMN overexpression promotes prognosis and chemosensitivity of triple negative breast cancer via acting as miR143-3p host gene and inhibiting DNA replication.

Journal of experimental & clinical cancer research : CR

Sheng X,Dai H,Du Y,Peng J,Sha R,Yang F,Zhou L,Lin Y,Xu S,Wu Y,Yin W,Lu J

Wed Jun 23 00:00:00 EDT 2021

METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.

Frontiers in oncology

Yang DD,Chen ZH,Yu K,Lu JH,Wu QN,Wang Y,Ju HQ,Xu RH,Liu ZX,Zeng ZL

Mon Sep 28 00:00:00 EDT 2020

MCMs expression in lung cancer: implication of prognostic significance.

Journal of Cancer

Liu YZ,Wang BS,Jiang YY,Cao J,Hao JJ,Zhang Y,Xu X,Cai Y,Wang MR

Fri Apr 08 00:00:00 EDT 2022

MCMs expression in lung cancer: implication of prognostic significance.

Journal of Cancer

Liu YZ,Wang BS,Jiang YY,Cao J,Hao JJ,Zhang Y,Xu X,Cai Y,Wang MR

Fri Apr 08 00:00:00 EDT 2022

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Cancer cytopathology

Liu YZ,Jiang YY,Wang BS,Hao JJ,Shang L,Zhang TT,Cao J,Xu X,Zhan QM,Wang MR

Sat Nov 01 00:00:00 EDT 2014

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Cancer cytopathology

Liu YZ,Jiang YY,Wang BS,Hao JJ,Shang L,Zhang TT,Cao J,Xu X,Zhan QM,Wang MR

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGC5315; minichromosome maintenance deficient 5 (cell division cycle 46); minichromosome maintenance deficient 5, cell division cycle 46; P1-CDC46

Gene Aliases: AA617332; AI324988; AL033333; CDC46; MCM5; Mcmd5; P1-CDC46

UniProt ID: (Human) P33992, (Mouse) P49718

Entrez Gene ID: (Human) 4174, (Rat) 291885, (Mouse) 17218

Molecular Function: DNA metabolism protein