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MCM6 Polyclonal Antibody

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MCM6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13347-2-AP (MCM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCM6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP ( MCM6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MCM6 Antibody in Western Blot (WB) — Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MCM6 Antibody in Immunoprecipitation (IP) — IP result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.

Product Details

13347-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2400	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCM6 Fusion Protein Ag4180 (471-821 aa encoded by BC032374) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: VAIHEAMEQ QTISITKAGV KATLNARTSI LAAANPISGH YDRSKSLKQN INLSAPIMSR FDLFFILVDE CNEVTDYAIA RRIVDLHSRI EESIDRVYSL DDIRRYLLFA RQFKPKISKE SEDFIVEQYK HLRQRDGSGV TKSSWRITVR QLESMIRLSE AMARMHCCDE VQPKHVKEAF RLLNKSIIRV ETPDVNLDQE EEIQMEVDEG AGGINGHADS PAPVNGINGY NEDINQESAP KASLRLGFSE YCRISNLIVL HLRKVEEEED ESALKRSELV NWYLKEIESE IDSEEELINK KRIIEKVIHR LTHYDHVLIE LTQAGLKGST EGSESYEEDP YLVVNPNYLL ED (471-821 aa encoded by BC032374)

Target Information

MCM6 may be involved in the control of a single round of DNA replication during S phase. It binds to chromatin during G1 and detaches from it during S phase as if it licenses the chromatin to replicate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MCMs expression in lung cancer: implication of prognostic significance.

Journal of Cancer

Liu YZ,Wang BS,Jiang YY,Cao J,Hao JJ,Zhang Y,Xu X,Cai Y,Wang MR

Fri Apr 08 00:00:00 EDT 2022

CtIP promotes G2/M arrest in etoposide-treated HCT116 cells in a p53-independent manner.

Journal of cellular physiology

Chen H,Shan J,Chen D,Wang R,Qi W,Wang H,Ke Y,Liu W,Zeng X

Mon Jul 01 00:00:00 EDT 2019

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Overexpression of MCM6 predicts poor survival in patients with glioma.

Human pathology

Cai HQ,Cheng ZJ,Zhang HP,Wang PF,Zhang Y,Hao JJ,Wang MR,Wan JH

Wed Aug 01 00:00:00 EDT 2018

MCM6 promotes metastasis of hepatocellular carcinoma via MEK/ERK pathway and serves as a novel serum biomarker for early recurrence.

Journal of experimental & clinical cancer research : CR

Liu M,Hu Q,Tu M,Wang X,Yang Z,Yang G,Luo R

Mon Jan 22 00:00:00 EST 2018

MCM6 promotes metastasis of hepatocellular carcinoma via MEK/ERK pathway and serves as a novel serum biomarker for early recurrence.

Journal of experimental & clinical cancer research : CR

Liu M,Hu Q,Tu M,Wang X,Yang Z,Yang G,Luo R

Mon Jan 22 00:00:00 EST 2018

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.

International journal of nanomedicine

Yuan Q,Han J,Cong W,Ge Y,Ma D,Dai Z,Li Y,Bi X

Tue Jun 02 00:00:00 EDT 2015

A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens.

Cancer cytopathology

Liu YZ,Jiang YY,Wang BS,Hao JJ,Shang L,Zhang TT,Cao J,Xu X,Zhan QM,Wang MR

Sat Nov 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: DNA replication licensing factor MCM6; intestinal DNA replication protein; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); mini chromosome maintenance deficient 6; minichromosome maintenance deficient (mis5, S. pombe) 6; minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); Mis5 homolog; p105MCM

Gene Aliases: ASP-l1; D1Wsu22e; MCG40308; MCM6; Mcmd6; Mis5; P105MCM

UniProt ID: (Human) Q14566, (Mouse) P97311

Entrez Gene ID: (Human) 4175, (Rat) 29685, (Mouse) 17219

Molecular Function: DNA metabolism protein