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MCM7 Polyclonal Antibody

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MCM7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 11225-1-AP (MCM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MCM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11225-1-AP ( MCM7 Antibody) at dilution of 1:200 (under 40x lens).

MCM7 Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11225-1-AP ( MCM7 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

MCM7 Antibody in Flow Cytometry (Flow) — 1X10^6 Raji cells were stained with 0.2ug MCM7 antibody (11225-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

MCM7 Antibody in Immunoprecipitation (IP) — IP result of anti-MCM7 (IP:11225-1-AP, 4ug; Detection:11225-1-AP 1:2000) with NIH/3T3 cells lysate 1600ug.

Product Details

11225-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MCM7 Fusion Protein Ag1730 (371-719 aa encoded by BC013375) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: IRGNINICL MGDPGVAKSQ LLSYIDRLAP RSQYTTGRGS SGVGLTAAVL RDSVSGELTL EGGALVLADQ GVCCIDEFDK MAEADRTAIH EVMEQQTISI AKAGILTTLN ARCSILAAAN PAYGRYNPRR SLEQNIQLPA ALLSRFDLLW LIQDRPDRDN DLRLAQHITY VHQHSRQPPS QFEPLDMKLM RRYIAMCREK QPMVPESLAD YITAAYVEMR REAWASKDAT YTSARTLLAI LRLSTALARL RMVDVVEKED VNEAIRLMEM SKDSLLGDKG QTARTQRPAD VIFATVRELV SGGRSVRFSE AEQRCVSRGF TPAQFQAALD EYEELNVWQV NASRTRITFV (371-719 aa encoded by BC013375)

Target Information

MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of Protein Abundance Changes in Hepatocellular Carcinoma Tissues Using PCT-SWATH.

Proteomics. Clinical applications

Zhu Y,Zhu J,Lu C,Zhang Q,Xie W,Sun P,Dong X,Yue L,Sun Y,Yi X,Zhu T,Ruan G,Aebersold R,Huang S,Guo T

Tue Jan 01 00:00:00 EST 2019

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Biomaterials

Song H,Guo T,Zhao Z,Wei Y,Luo H,Weng W,Zhang R,Zhong M,Chen C,Su J,Shen W

Sat Sep 01 00:00:00 EDT 2018

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Oncotarget

Ma Y,Fei L,Zhang M,Zhang W,Liu X,Wang C,Luo Y,Zhang H,Han Y

Fri Dec 01 00:00:00 EST 2017

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Oncotarget

Fan L,Cao X,Yan H,Wang Q,Tian X,Zhang L,He X,Borjihan G

Tue Jul 18 00:00:00 EDT 2017

Subcellular quantitative proteomics reveals multiple pathway cross-talk that coordinates specific signaling and transcriptional regulation for the early host response to LPS.

Journal of proteome research

Du R,Long J,Yao J,Dong Y,Yang X,Tang S,Zuo S,He Y,Chen X

Mon Apr 05 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CDC47 homolog; DNA replication licensing factor MCM7; homolog of S. cerevisiae Cdc47; mini chromosome maintenance deficient 7; minichromosome maintenance deficient 7; minichromosome maintenance protein 7; P1 P85MCM; P1.1-MCM3; protein phosphatase 1, regulatory subunit 104

Gene Aliases: AI747533; CDC47; mCDC47; MCM2; MCM7; Mcmd7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104

UniProt ID: (Human) P33993, (Mouse) Q61881

Entrez Gene ID: (Human) 4176, (Mouse) 17220, (Rat) 288532

Molecular Function: DNA metabolism protein

Function(s)

G1/S transition of mitotic cell cycle DNA replication DNA unwinding involved in DNA replication DNA replication initiation cellular response to DNA damage stimulus cell proliferation regulation of phosphorylation response to drug cellular response to epidermal growth factor stimulus cellular response to xenobiotic stimulus cell cycle regulation of transcription, DNA-templated cellular response to organic substance

Process(es)

DNA binding single-stranded DNA binding ATP-dependent DNA helicase activity protein binding ATP binding nucleotide binding DNA helicase activity helicase activity hydrolase activity DNA-dependent ATPase activity

MCM7 Polyclonal Antibody

Product Details

11225-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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