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Alomone Labs, LTD
MCU Polyclonal Antibody
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FIGURE: 1 / 2
MCU Antibody (ACC-328-200UL) in IHC (F)
Expression of MCU in mouse midbrain. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MCU Antibody (#ACC-328), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the mouse substantia nigra region, showed immunoreactivity (green) in neuronal profiles in the substantia nigra pars compacta (SNC, horizontal arrows). B. Pre-incubation of the Antibody with MC... View More
Product Details
ACC-328-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter, N-terminus, mitochondrial matrix
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Amount
160 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium uniporter protein, mitochondrial; coiled-coil domain containing 109A; Coiled-coil domain-containing protein 109A; HsMCU; unnamed protein product
Gene Aliases: 2010012O16Rik; AV064928; C10orf42; CCDC109A; D130073L02Rik; Gm64; HsMCU; MCU
UniProt ID: (Human) Q8NE86, (Mouse) Q3UMR5
Entrez Gene ID: (Human) 90550, (Rat) 294560, (Mouse) 215999
mitochondrial calcium ion transport
calcium-mediated signaling
positive regulation of insulin secretion
protein complex oligomerization
calcium ion transmembrane import into mitochondrion
glucose homeostasis
mitochondrial calcium ion homeostasis
positive regulation of mitochondrial calcium ion concentration
generation of precursor metabolites and energy
ion transport
calcium ion transport
cell proliferation
cell migration
ion transmembrane transport
protein oligomerization
calcium ion transmembrane transport
cellular response to calcium ion starvation
positive regulation of neutrophil chemotaxis
positive regulation of mitochondrial fission
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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