Invitrogen

MDA5 Polyclonal Antibody

Product Image Gallery

MDA5 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of MDA5 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with MDA5 polyclonal antibody (Product # PA5-102605) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

MDA5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded MDA5 in mouse testis tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with MDA5 polyclonal antibody (Product # PA5-102605) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

MDA5 Antibody in Western Blot (WB) — Western blot analysis of MDA5 in Jurkat cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with MDA5 polyclonal antibody (Product # PA5-102605).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IFIH1, corresponding to a region within C-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2852007

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; Interferon induced with helicase C domain protein 1; interferon induced, with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation associated protein-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116

Gene Aliases: 9130009C22Rik; AGS7; Helicard; Hlcd; IDDM19; IFIH1; MDA-5; MDA5; RGD1560496; RH116; RLR-2; SGMRT1

UniProt ID: (Human) Q9BYX4, (Mouse) Q8R5F7

Entrez Gene ID: (Human) 64135, (Rat) 499801, (Mouse) 71586

Function(s)