Invitrogen

MDA5 Polyclonal Antibody

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MDA5 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of MDA5 in human placenta using a MDA5 polyclonal antibody (Product # PA5-82028). The analysis shows moderate cytoplasmic positivity in trophoblastic cells.

MDA5 Antibody in Western Blot (WB) — Western blot analysis of MDA5 by a MDA5 polyclonal antibody (Product # PA5-82028). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line SK-MEL-30.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human IFIH1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2789189

Product Specific Information

Immunogen sequence: TIRMIDAYTH LETFYNEEKD KKFAVIEDDS DEGGDDEYCD GDEDEDDLKK PLKLDETDRF LMTLFFENNK MLKRLAENPE YENEKLTKLR NTIMEQYTRT EESARGIIFT KTRQSAYALS QWITENEKFA EVGVKAHHLI GAGHSSEFKP

Target Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp and a protein kinase C-activating compound, mezerein. Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CADM-140 autoantigen; Clinically amyopathic dermatomyositis autoantigen 140 kDa; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; Helicard; Helicase with 2 CARD domains; interferon induced, with helicase C domain 1; Interferon-induced helicase C domain-containing protein 1; Interferon-induced with helicase C domain protein 1; MDA-5; melanoma differentiation associated protein-5; melanoma differentiation-associated gene 5; Melanoma differentiation-associated protein 5; MGC133047; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116

Gene Aliases: AGS7; Hlcd; IDDM19; IFIH1; MDA-5; MDA5; RH116; RLR-2; SGMRT1

UniProt ID: (Human) Q9BYX4

Entrez Gene ID: (Human) 64135

Function(s)

detection of virus response to virus protein sumoylation negative regulation of type I interferon production positive regulation of interferon-alpha production positive regulation of interferon-beta production regulation of type III interferon production cytoplasmic pattern recognition receptor signaling pathway in response to virus MDA-5 signaling pathway innate immune response

Process(es)

DNA binding double-stranded RNA binding single-stranded RNA binding helicase activity protein binding ATP binding zinc ion binding ribonucleoprotein complex binding