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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500- 1:2,000 | - |
ELISA (ELISA) |
1:10,000-1:50,000 | - |
Immunoprecipitation (IP) |
1:500- 1:2,000 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 679-694 of Human MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 is hyper-phosphorylated in an ATM-dependent manner. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
Contains |
0.01% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
0
MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1
Gene Aliases: KIAA0170; MDC1; NFBD1
UniProt ID: (Human) Q14676
Entrez Gene ID: (Human) 9656
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