Invitrogen

MDC1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (19) MDC1 antibodies

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Datasheet

Protocols

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MDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Mediator of DNA damage checkpoint protein 1 was performed using 70% confluent log phase HCT 116 Nocodazole treated (200 nmol/L for 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MDC1 Polyclonal Antibody (Product # PA5-79652) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear foci localization of MDC1 on Nocodazole treatment. Panel e represents untreated cells with nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MDC1 on paraffin-embedded human colon cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with MDC1 polyclonal antibody (Product# PA5-79652) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

MDC1 Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using MDC1 Polyclonal Antibody (Product # PA5-79652), shows nuclear foci localization on Nocodazole treatment. {TM}

Product Details

PA5-79652

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived human MDC1 recombinant protein (Position: A534-Q626). View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 4mg trehalose
Contains	0.05mg sodium azide
Storage conditions	-20°C
RRID	AB_2746767

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

MDC1 participates in the early response to DNA damage. It is thought to be a member of the BRCT (BRCA1 C-terminus) super family of nuclear proteins. It contains an N-terminus forkhead-associated (FHA) motif, two C-terminus BRCT motifs and 13 internal repetitions of a 41 amino acid sequence.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DAAP-285E11.7; DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; Nuclear factor with BRCT domains 1

Gene Aliases: KIAA0170; MDC1; NFBD1

UniProt ID: (Human) Q14676

Entrez Gene ID: (Human) 9656

Function(s)

double-strand break repair via nonhomologous end joining protein sumoylation intra-S DNA damage checkpoint

Process(es)

protein binding protein C-terminus binding FHA domain binding