Keep as concentrated solution.
Positive Control: Jurkat, Raji, NCI-H929, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, HepG2, HepG2 (starvation and 1 uM Staurosporine treatment for 24 hr), MDM2-transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
MDM2 is a ubiquitin ligase for p53 and plays a central role in regulation of the stability of p53. MDM2 is located on chromosome 12 on the q arm. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. MDM2 has also been shown to negatively regulate p53 function. MDM2 binds and inhibits transactivation role played by p53 and overexpression of MDM2 can result in the inactivation of p53 and decrease its tumor suppressor function. Another process by which MDM2 can inactivate p53 is by degrading p53 as the protein also possesses E3 ubiquitin ligase activity. Further, MDM2 plays important roles in apoptosis and cell cycle. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. In addition to p53, MDM2 is involved in processes of cell cycle, apoptosis, and tumorigenesis through interactions with proteins that include retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants of MDM2 have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: double minute 2; Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; E3 ubiquitin-protein ligase Mdm2 antibody; Hdm2; hdm2 antibody; HDMX antibody; human homolog of; Mdm2; MDM2 antibody; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog antibody; MDM2 proto-oncogene, E3 ubiquitin protein ligase; MDM2 variant FB28 antibody; MDM2 variant FB30 antibody; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370 antibody; MGC71221 antibody; Oncoprotein Mdm2; oncoprotein Mdm2 antibody; p53 binding protein antibody; p53-binding protein antibody; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; transformed 3T3 cell double minute 2
Gene Aliases: 1700007J15Rik; AA415488; ACTFS; hdm2; HDMX; Mdm-2; MDM2