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Invitrogen
MED14 Polyclonal Antibody
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FIGURE: 1 / 1
MED14 Antibody (PA5-79655) in WB
Western blot analysis of MED14 in Lane 1: rat liver tissue lysate, Lane 2: mouse liver tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with MED14 polyclonal antibody (Product # PA5-79655) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat... View More
Product Details
PA5-79655
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MED14 recombinant protein (Position: D181-D375).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat liver tissue, mouse liver tissue.
Target Information
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activator-recruited cofactor 150 kDa component; ARC150; Cofactor required for Sp1 transcriptional activation subunit 2; cofactor required for Sp1 transcriptional activation subunit 2 (150 kDa); cofactor required for Sp1 transcriptional activation, subunit 2; cofactor required for Sp1 transcriptional activation, subunit 2 (150kD); CRSP complex subunit 2; DRIP150; EXLM1; hRGR1; human homolog of yeast RGR1; Mediator complex subunit 14; Mediator of RNA polymerase II transcription subunit 14; MGC104513; RGR1 homolog; Thyroid hormone receptor-associated protein complex 170 kDa component; thyroid hormone receptor-associated protein complex component TRAP170; transcriptional co-activator CRSP150; Transcriptional coactivator CRSP150; Trap170; unnamed protein product; vitamin D receptor-interacting protein complex component DRIP150; Vitamin D3 receptor-interacting protein complex 150 kDa component
Gene Aliases: 9930001L01Rik; ARC150; AU041628; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; ENSMUSG00000073278; EXLM1; Gm641; MED14; ORF1; RGD1560170; RGR1; TRAP170
UniProt ID: (Human) O60244, (Mouse) A2ABV5
Entrez Gene ID: (Human) 9282, (Mouse) 26896, (Rat) 317343
regulation of transcription from RNA polymerase II promoter
positive regulation of transcription elongation from RNA polymerase II promoter
somatic stem cell population maintenance
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription initiation from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase II promoter
stem cell population maintenance
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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