Proteintech

MEK2 Polyclonal Antibody

MEK2 Antibody in Immunocytochemistry (ICC/IF)

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MEK2 Antibody (11049-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MEK2 Antibody in Immunoprecipitation (IP)

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Product Details

11049-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 38 publications 38 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MEK2 Fusion Protein Ag1481 (1-400 aa encoded by BC018645)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

11049-1-AP can recognize both MEK2 and MEK1.

Immunogen sequence: MLARRKPVL PALTINPTIA EGPSPTSEGA SEANLVDLQK KLEELELDEQ QKKRLEAFLT QKAKVGELKD DDFERISELG AGNGGVVTKV QHRPSGLIMA RKLIHLEIKP AIRNQIIREL QVLHECNSPY IVGFYGAFYS DGEISICMEH MDGGSLDQVL KEAKRIPEEI LGKVSIAVLR GLAYLREKHQ IMHRDVKPSN ILVNSRGEIK LCDFGVSGQL IDSMANSFVG TRSYMAPERL QGTHYSVQSD IWSMGLSLVE LAVGRYPIPP PDAKELEAIF GRPVVDGEEG EPHSISPRPR PPGRPVSGHG MDSRPAMAIF ELLDYIVNEP PPKLPNGVFT PDFQEFVNKC LIKNPAERAD LKMLTNHTFI KRSEVEEVDF AGWLCKTLRL NQPGTPTRTA V (1-400 aa encoded by BC018645)

Target Information

MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that is known to play a critical role in mitogen growth factor signal transduction. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; MAPK; MAPK/ERK kinase 1; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 1; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1; protein kinase, mitogen activated, kinase 2, p45

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Gene Aliases: AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2

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UniProt ID: (Human) P36507, (Mouse) Q63932

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Entrez Gene ID: (Human) 5605, (Mouse) 26396

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Function(s)

protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity MAP kinase kinase activity protein serine/threonine/tyrosine kinase activity protein tyrosine kinase activity protein binding ATP binding PDZ domain binding protein complex scaffold protein serine/threonine kinase activator activity metal ion binding scaffold protein binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

MAPK cascade activation of MAPK activity heart development negative regulation of gene expression peptidyl-tyrosine phosphorylation thyroid gland development regulation of stress-activated MAPK cascade proteolysis in other organism peptidyl-serine autophosphorylation positive regulation of transcription, DNA-templated thymus development regulation of axon regeneration positive regulation of axonogenesis face development trachea formation epithelial cell proliferation involved in lung morphogenesis ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of protein serine/threonine kinase activity regulation of Golgi inheritance positive regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of cell motility regulation of early endosome to late endosome transport regulation of protein phosphorylation protein phosphorylation phosphorylation lung morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MEK2 Polyclonal Antibody

MEK2 Antibody (11049-1-AP) in ICC/IF

Product Details

11049-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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