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Product Details
11049-1-AP
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 3 publications 3 publications |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
MEK2 Fusion Protein Ag1481 (1-400 aa encoded by BC018645) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.27 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Product Specific Information
11049-1-AP can recognize both MEK2 and MEK1.
Immunogen sequence: MLARRKPVL PALTINPTIA EGPSPTSEGA SEANLVDLQK KLEELELDEQ QKKRLEAFLT QKAKVGELKD DDFERISELG AGNGGVVTKV QHRPSGLIMA RKLIHLEIKP AIRNQIIREL QVLHECNSPY IVGFYGAFYS DGEISICMEH MDGGSLDQVL KEAKRIPEEI LGKVSIAVLR GLAYLREKHQ IMHRDVKPSN ILVNSRGEIK LCDFGVSGQL IDSMANSFVG TRSYMAPERL QGTHYSVQSD IWSMGLSLVE LAVGRYPIPP PDAKELEAIF GRPVVDGEEG EPHSISPRPR PPGRPVSGHG MDSRPAMAIF ELLDYIVNEP PPKLPNGVFT PDFQEFVNKC LIKNPAERAD LKMLTNHTFI KRSEVEEVDF AGWLCKTLRL NQPGTPTRTA V (1-400 aa encoded by BC018645)
Target Information
MEK1 (Mitogen activated protein kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; MAPK; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1; protein kinase, mitogen activated, kinase 2, p45
Gene Aliases: AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2
UniProt ID: (Human) P36507, (Mouse) Q63932
Entrez Gene ID: (Human) 5605, (Mouse) 26396
Molecular Function:
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPK activity
heart development
negative regulation of gene expression
peptidyl-tyrosine phosphorylation
thyroid gland development
regulation of stress-activated MAPK cascade
proteolysis in other organism
peptidyl-serine autophosphorylation
positive regulation of transcription, DNA-templated
thymus development
regulation of axon regeneration
positive regulation of axonogenesis
face development
trachea formation
epithelial cell proliferation involved in lung morphogenesis
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of protein serine/threonine kinase activity
regulation of Golgi inheritance
positive regulation of production of miRNAs involved in gene silencing by miRNA
positive regulation of cell motility
regulation of early endosome to late endosome transport
regulation of protein phosphorylation
protein phosphorylation
phosphorylation
lung morphogenesis
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase activity
protein serine/threonine/tyrosine kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
PDZ domain binding
protein complex scaffold
protein serine/threonine kinase activator activity
metal ion binding
scaffold protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
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