Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 3 publications 3 publications |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
MEK2 Fusion Protein Ag1481 (1-400 aa encoded by BC018645) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.27 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
11049-1-AP can recognize both MEK2 and MEK1.
Immunogen sequence: MLARRKPVL PALTINPTIA EGPSPTSEGA SEANLVDLQK KLEELELDEQ QKKRLEAFLT QKAKVGELKD DDFERISELG AGNGGVVTKV QHRPSGLIMA RKLIHLEIKP AIRNQIIREL QVLHECNSPY IVGFYGAFYS DGEISICMEH MDGGSLDQVL KEAKRIPEEI LGKVSIAVLR GLAYLREKHQ IMHRDVKPSN ILVNSRGEIK LCDFGVSGQL IDSMANSFVG TRSYMAPERL QGTHYSVQSD IWSMGLSLVE LAVGRYPIPP PDAKELEAIF GRPVVDGEEG EPHSISPRPR PPGRPVSGHG MDSRPAMAIF ELLDYIVNEP PPKLPNGVFT PDFQEFVNKC LIKNPAERAD LKMLTNHTFI KRSEVEEVDF AGWLCKTLRL NQPGTPTRTA V (1-400 aa encoded by BC018645)
MEK1 (Mitogen activated protein kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; ERK kinase 2; FLJ26075; MAP kinase kinase 2; MAP kinase/Erk kinase; MAP2K1; MAPK; MAPK/ERK kinase 2; MAPKK 1; MAPKK 2; MEK 2; MEK-1; MEK-2; mitogen activated protein kinase kinase 2; mitogen-activated protein kinase kinase 2, p45; MKK1; OTTHUMP00000165827; PRKMK1; protein kinase, mitogen activated, kinase 2, p45
Gene Aliases: AA589381; CFC4; MAP2K2; MAPKK2; MEK2; MK2; MKK2; PRKMK2
UniProt ID: (Human) P36507, (Mouse) Q63932
Entrez Gene ID: (Human) 5605, (Mouse) 26396
Molecular Function:
non-receptor serine/threonine protein kinase
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