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Invitrogen
MEK3 Polyclonal Antibody
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FIGURE: 1 / 10
MEK3 Antibody (PA5-94973) in IHC (P)
Immunohistochemical analysis of MAP2K3 in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MAP2K3 antibody (Product # PA5-94973) overnight at 4°C. Biotinylated goat anti-rabbit IgG was us... View More
Product Details
PA5-94973
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3 (320-334aa MEHPFFTLHKTKKTD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human CACO-2 whole cell, human HEK293 whole cell, human HEPG2 whole cell, human PC-3 whole cell, human HELA whole cell, human THP-1 whole cell, rat brain tissue, rat heart tissue, rat NRK whole cell, mouse Neuro-2a whole cell, mouse HEPA1-6 whole cell. IHC: mouse brain tissue, rat brain tissue, human intestine cancer tissue, rat intestine tissue, human lung cancer tissue, human breast cancer tissue, mouse intestine tissue. Flow: A549 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
MAP2K3 (MEK3) is a dual-specificity protein kinase which acts in cellular signal transduction pathways, and is necessary for the expression of glucose transporter. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for MEK3.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 3; EC 2.7.12.2; MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK 3; mitogen activated protein kinase kinase 3; protein kinase, mitogen-activated, kinase 3; SAPK kinase 2; Stress-activated protein kinase kinase 2; unnamed protein product
Gene Aliases: AW212142; MAP2K3; MAPKK3; MEK3; MKK3; mMKK3b; PRKMK3; SAPKK-2; SAPKK2; SKK2
UniProt ID: (Human) P46734, (Mouse) O09110
Entrez Gene ID: (Human) 5606, (Mouse) 26397, (Rat) 303200
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase activity
protein tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
regulation of cytokine production
response to ischemia
inflammatory response
signal transduction
heart development
stress-activated protein kinase signaling cascade
negative regulation of hippo signaling
cellular response to vascular endothelial growth factor stimulus
p38MAPK cascade
positive regulation of MAPK cascade
positive regulation of blood vessel endothelial cell migration
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
protein maturation
cardiac muscle contraction
pyroptosis
cellular response to lipopolysaccharide
cellular response to UV-B
cellular response to sorbitol
cellular senescence
regulation of intracellular signal transduction
NLRP1 inflammasome complex assembly
activation of MAPK activity
protein phosphorylation
regulation of mitotic cell cycle
phosphorylation
regulation of cytokine biosynthetic process
regulation of apoptotic process
positive regulation of protein phosphorylation
positive regulation of nitric-oxide synthase biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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