Invitrogen

MEK5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (26) MEK5 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

MEK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

MEK5 Antibody (PA5-29236) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MEK5 (Product # PA5-29236) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

MEK5 Antibody in Western Blot (WB) — Western Blot using MEK5 Polyclonal Antibody (Product # PA5-29236). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 10% SDS PAGE. MEK5 Polyclonal Antibody (Product # PA5-29236) diluted at 1:2,000.

MEK5 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni's post hoc comparison against control conditions

MEK5 Antibody — Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni's post hoc comparison against control conditions {TM}

Product Details

PA5-29236

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 205 of Human MEK5

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546712

Product Specific Information

Recommended positive controls: U87-MG.

Predicted reactivity: Mouse (98%), Rat (98%), Chicken (91%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP2K5 (MEK5) serine/threonine kinase (mitogen-activated protein kinase 5) or MEK5 is a dual specificity protein kinase that belongs to the MAP kinase family which specifically interacts with and activates MAPK7/ERK5. MAP2K5 itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation. The rs3743354 polymorphism in the MAP2K5 promoter may affect the risk of developing colorectal cancer (CRC).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro.

Journal of neuroinflammation

Bortell N,Basova L,Semenova S,Fox HS,Ravasi T,Marcondes MC

PA529236 was used in immunocytochemistry and western blot to assess characteristics of the astrocytic acute response to methamphetamine

Thu Mar 09 00:00:00 EST 2017