Invitrogen

MEK5 Polyclonal Antibody

Product Image Gallery

MEK5 Antibody — Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni and apos;s post hoc comparison against control conditions

MEK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of MEK5/MAP2K5 in paraffin-embedded breast carcinoma using a MEK5/MAP2K5 polyclonal antibody (Product # PA5-29236) at a dilution of 1:250.

MEK5 Antibody in Western Blot (WB) — Western blot analysis of MEK5/MAP2K5 using 30 µg of U87-MG lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MEK5/MAP2K5 polyclonal antibody (Product # PA5-29236) at a dilution of 1:2000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Published Applications

Western Blot (WB)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 205 of Human MEK5

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2546712

Product Specific Information

PA5-29236 targets MEK5 in IHC (P) and WB applications and shows reactivity with Human samples.

The PA5-29236 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 205 of Human MEK5.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 5; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; MEK5; PRKMK5

Gene Aliases: HsT17454; MAP2K5; MAPKK5; MEK5; MKK5; PRKMK5

UniProt ID: (Human) Q13163

Entrez Gene ID: (Human) 5607

Function(s)