Invitrogen

MEK5 Polyclonal Antibody

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (26) MEK5 antibodies

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Datasheet

Protocols

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MEK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of MEK5/MAP2K5 in paraffin-embedded breast carcinoma using a MEK5/MAP2K5 polyclonal antibody (Product # PA5-29236) at a dilution of 1:250.

MEK5 Antibody in Western Blot (WB) — Western blot analysis of MEK5/MAP2K5 using 30 µg of U87-MG lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MEK5/MAP2K5 polyclonal antibody (Product # PA5-29236) at a dilution of 1:2000.

MEK5 Antibody — Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni's post hoc comparison against control conditions {TM}

MEK5 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 2 Validation of selected upregulated genes. a Transcriptional levels of MAP2K5, GRP65, and CXCL5 in astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h, examined by SyBrGreen qRT-PCR. Results are the Mean +- SEM of three independent experiments performed in duplicate. b Representative western blots for detection of MEK5, the protein encoded by MAP2K5 gene, GPR65, and normalizing b-actin, in protein extracts from astrocytes stimulated with 1, 10, and 100 muM of Meth for 24 h. c Normalized band intensity was calculated in ImageJ software (NIH). d Confocal imaging showing representative astrocytic cultures stained with specific antibodies for detection of MEK5, GPR65, and CXCL5 in 10 and 100 muM Meth treatments. e Fluorescence intensity of the expression of MEK5, GPR65, and CXCL5 was calculated in ImageJ (NIH) for cultures stimulated with 1, 10, and 100 muM, as well as controls. * p < 0.05 in one-way ANOVA followed by Bonferroni's post hoc comparison against control conditions

Product Details

PA5-29236

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 205 of Human MEK5 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546712

Product Specific Information

Recommended positive controls: U87-MG.

Predicted reactivity: Mouse (98%), Rat (98%), Chicken (91%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MAP2K5 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. It specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by MAP2K5 is involved in growth factor stimulated cell proliferation and muscle cell differentiation.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro.

Journal of neuroinflammation

Bortell N,Basova L,Semenova S,Fox HS,Ravasi T,Marcondes MC

PA529236 was used in immunocytochemistry and western blot to assess characteristics of the astrocytic acute response to methamphetamine

Thu Mar 09 00:00:00 EST 2017

Astrocyte-specific overexpressed gene signatures in response to methamphetamine exposure in vitro.

Journal of neuroinflammation

Bortell N,Basova L,Semenova S,Fox HS,Ravasi T,Marcondes MC

PA529236 was used in immunocytochemistry and western blot to assess characteristics of the astrocytic acute response to methamphetamine

Thu Mar 09 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Dual specificity mitogen-activated protein kinase kinase 5; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAPK/ERK kinase 5; MAPKK 5; MEK 5

Gene Aliases: HsT17454; MAP2K5; MAPKK5; MEK5; MKK5; PRKMK5

UniProt ID: (Human) Q13163

Entrez Gene ID: (Human) 5607

Molecular Function: non-receptor serine/threonine protein kinase