Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:100 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1). |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.53 mg/mL |
Purification |
Affinity Chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.01% thimerosal |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2855344 |
Sequence of this protein is as follows: TGAGEFQGQL LGTIAFMAPE VLRGQQYGRS CDVWSVGCAI IEMACAKPPW NAEKHSNHLA LIFKIASATT APSIPSHLSP GLRDVALRCL ELQPQDRPPS RELLKHPVFR TTW
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; mitogen activated protein kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
Gene Aliases: MAP3K1; MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6
UniProt ID: (Human) Q13233, (Rat) Q62925
Entrez Gene ID: (Human) 4214, (Mouse) 26401, (Rat) 116667
Molecular Function:
non-receptor serine/threonine protein kinase
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