Invitrogen

MEKK1 Polyclonal Antibody

Product Image Gallery

MEKK1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of MEKK1 in L929 cells. Samples were incubated in polyclonal MEKK1 antibody (Product # PA5-109933) using a dilution of 1:100, followed by DAPI.

MEKK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MEKK1 in paraffin-embedded mouse kidney tissue. Samples were incubated in polyclonal MEKK1 antibody (Product # PA5-109933) using a dilution of 1:100.

MEKK1 Antibody in Western Blot (WB) — Western blot analysis of MEKK1 in A-431, Raji, HeLa, mouse spleen. Samples were incubated in polyclonal MEKK1 antibody (Product # PA5-109933) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1400 to the C-terminus of human MAP3K1 (NP_005912.1).

Conjugate

Unconjugated

Form

Liquid

Concentration

2.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2855344

Product Specific Information

Sequence of this protein is as follows: TGAGEFQGQL LGTIAFMAPE VLRGQQYGRS CDVWSVGCAI IEMACAKPPW NAEKHSNHLA LIFKIASATT APSIPSHLSP GLRDVALRCL ELQPQDRPPS RELLKHPVFR TTW

Target Information

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK1 can phosphorylate and activate MAPKK 1 and MAPKK 2 (MEK1/MEK2) which leads to phosphorylation of MAP kinases. It is also a highly efficient activator of the JNK cascade. The protein contains a putative 1 RING-type zinc finger and 1 SWIM-type zinc finger.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEK kinase 1; MEKK 1; mitogen activated protein kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase

Gene Aliases: MAP3K1; MAPKKK1; MEKK; MEKK 1; MEKK1; SRXY6

UniProt ID: (Human) Q13233, (Rat) Q62925

Entrez Gene ID: (Human) 4214, (Mouse) 26401, (Rat) 116667

Molecular Function: non-receptor serine/threonine protein kinase