Invitrogen

METTL1 Polyclonal Antibody

View all (12) METTL1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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METTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of human METTL1 in human bladder carcinoma with METTL1 Polyclonal Antibody (Product # PA5-80810, 1:5,000 dilution, formalin-fixed paraffin embedded sections). Left panel: tissue incubated with primary antibody. Right panel: tissue incubated with mixture of primary antibody and antigen (recombinant protein).

METTL1 Antibody in Western Blot (WB) — Western Blot using METTL1 Polyclonal Antibody (Product # PA5-80810) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).

METTL1 Antibody in Immunoprecipitation (IP) — METTL1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 0.5 µL with METTL1 Polyclonal Antibody (Product # PA5-80810) and 15 µL of 50 % Protein G agarose. Primary antibody: METTL1 Polyclonal Antibody, at 1:1,000 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.

Product Details

PA5-80810

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:2,000-1:10,000	-
ELISA (ELISA)	1:25000-1:50000	-
Immunoprecipitation (IP)	0.2-1 µL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human METTL1 protein (Asp32-Gln265) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	no preservative
Storage conditions	Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID	AB_2788084

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human METTL1.

Target Information

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: D1075-like gene product; Methyltransferase-like protein 1; METTL1; miRNA (guanine-N(7)-)-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase

Gene Aliases: C12orf1; METTL1; TRM8; TRMT8; YDL201w

UniProt ID: (Human) Q9UBP6

Entrez Gene ID: (Human) 4234

Molecular Function: RNA processing factor

Function(s)

tRNA modification tRNA methylation RNA (guanine-N7)-methylation

Process(es)

tRNA binding protein binding tRNA (guanine-N7-)-methyltransferase activity

METTL1 Polyclonal Antibody

Product Details

PA5-80810

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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