Proteintech

METTL3 Polyclonal Antibody

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METTL3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 15073-1-AP ( METTL3 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

METTL3 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

METTL3 Antibody in Immunoprecipitation (IP) — IP result of anti-METTL3 (IP:15073-1-AP, 4 ug; Detection:15073-1-AP 1:300) with HEK-293 cells lysate 3000 ug.

Product Details

15073-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 37 publications 37 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	-	View 5 publications 5 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Fruit fly, Human, Mouse, Zebrafish
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	METTL3 Fusion Protein Ag7110 (229-580 aa encoded by BC001650) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LNQQSTKEQ QSKKVSQEIL ELLNTTTAKE QSIVEKFRSR GRAQVQEFCD YGTKEECMKA SDADRPCRKL HFRRIINKHT DESLGDCSFL NTCFHMDTCK YVHYEIDACM DSEAPGSKDH TPSQELALTQ SVGGDSSADR LFPPQWICCD IRYLDVSILG KFAVVMADPP WDIHMELPYG TLTDDEMRRL NIPVLQDDGF LFLWVTGRAM ELGRECLNLW GYERVDEIIW VKTNQLQRII RTGRTGHWLN HGKEHCLVGV KGNPQGFNQG LDCDVIVAEV RSTSHKPDEI YGMIERLSPG TRKIELFGRP HNVQPNWITL GNQLDGIHLL DPDVVARFKQ RYPDGIISKP KNL (229-580 aa encoded by BC001650)

Target Information

METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HIV-1 envelope proteins up-regulate N ⁶-methyladenosine levels of cellular RNA independently of viral replication.

The Journal of biological chemistry

Tirumuru N,Wu L

Fri Mar 01 00:00:00 EST 2019

m⁶A modification controls the innate immune response to infection by targeting type I interferons.

Nature immunology

Winkler R,Gillis E,Lasman L,Safra M,Geula S,Soyris C,Nachshon A,Tai-Schmiedel J,Friedman N,Le-Trilling VTK,Trilling M,Mandelboim M,Hanna JH,Schwartz S,Stern-Ginossar N

Fri Feb 01 00:00:00 EST 2019

m⁶A modification controls the innate immune response to infection by targeting type I interferons.

Nature immunology

Winkler R,Gillis E,Lasman L,Safra M,Geula S,Soyris C,Nachshon A,Tai-Schmiedel J,Friedman N,Le-Trilling VTK,Trilling M,Mandelboim M,Hanna JH,Schwartz S,Stern-Ginossar N

Fri Feb 01 00:00:00 EST 2019

Mettl3 Deficiency Sustains Long-Chain Fatty Acid Absorption through Suppressing Traf6-Dependent Inflammation Response.

Journal of immunology (Baltimore, Md. : 1950)

Zong X,Zhao J,Wang H,Lu Z,Wang F,Du H,Wang Y

Tue Jan 15 00:00:00 EST 2019

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

Nucleic acids research

Hao H,Hao S,Chen H,Chen Z,Zhang Y,Wang J,Wang H,Zhang B,Qiu J,Deng F,Guan W

Thu Jan 10 00:00:00 EST 2019

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

Nucleic acids research

Hao H,Hao S,Chen H,Chen Z,Zhang Y,Wang J,Wang H,Zhang B,Qiu J,Deng F,Guan W

Thu Jan 10 00:00:00 EST 2019

RNA m⁶ A modification enzymes shape innate responses to DNA by regulating interferon β.

Genes & development

Rubio RM,Depledge DP,Bianco C,Thompson L,Mohr I

Sat Dec 01 00:00:00 EST 2018

Mettl3-mediated m⁶A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis.

Nature communications

Wu Y,Xie L,Wang M,Xiong Q,Guo Y,Liang Y,Li J,Sheng R,Deng P,Wang Y,Zheng R,Jiang Y,Ye L,Chen Q,Zhou X,Lin S,Yuan Q

Wed Nov 14 00:00:00 EST 2018

Mettl3-mediated m⁶A RNA methylation regulates the fate of bone marrow mesenchymal stem cells and osteoporosis.

Nature communications

Wu Y,Xie L,Wang M,Xiong Q,Guo Y,Liang Y,Li J,Sheng R,Deng P,Wang Y,Zheng R,Jiang Y,Ye L,Chen Q,Zhou X,Lin S,Yuan Q

Wed Nov 14 00:00:00 EST 2018

Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m⁶A mRNA Methylation.

Cell reports

Zhong X,Yu J,Frazier K,Weng X,Li Y,Cham CM,Dolan K,Zhu X,Hubert N,Tao Y,Lin F,Martinez-Guryn K,Huang Y,Wang T,Liu J,He C,Chang EB,Leone V

Tue Nov 13 00:00:00 EST 2018

Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m⁶A mRNA Methylation.

Cell reports

Zhong X,Yu J,Frazier K,Weng X,Li Y,Cham CM,Dolan K,Zhu X,Hubert N,Tao Y,Lin F,Martinez-Guryn K,Huang Y,Wang T,Liu J,He C,Chang EB,Leone V

Tue Nov 13 00:00:00 EST 2018

METTL3 promotes ovarian carcinoma growth and invasion through the regulation of AXL translation and epithelial to mesenchymal transition.

Gynecologic oncology

Hua W,Zhao Y,Jin X,Yu D,He J,Xie D,Duan P

Thu Nov 01 00:00:00 EDT 2018

METTL3 promotes ovarian carcinoma growth and invasion through the regulation of AXL translation and epithelial to mesenchymal transition.

Gynecologic oncology

Hua W,Zhao Y,Jin X,Yu D,He J,Xie D,Duan P

Thu Nov 01 00:00:00 EDT 2018

A dynamic N⁶-methyladenosine methylome regulates intrinsic and acquired resistance to tyrosine kinase inhibitors.

Cell research

Yan F,Al-Kali A,Zhang Z,Liu J,Pang J,Zhao N,He C,Litzow MR,Liu S

Thu Nov 01 00:00:00 EDT 2018

mRNA m⁶A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes.

International journal of obesity (2005)

Wang X,Sun B,Jiang Q,Wu R,Cai M,Yao Y,Liu Q,Shi H,Feng J,Wang Y

Thu Nov 01 00:00:00 EDT 2018

The m⁶A-methylase complex recruits TREX and regulates mRNA export.

Scientific reports

Lesbirel S,Viphakone N,Parker M,Parker J,Heath C,Sudbery I,Wilson SA

Fri Sep 14 00:00:00 EDT 2018

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

Nature

Choe J,Lin S,Zhang W,Liu Q,Wang L,Ramirez-Moya J,Du P,Kim W,Tang S,Sliz P,Santisteban P,George RE,Richards WG,Wong KK,Locker N,Slack FJ,Gregory RI

Sat Sep 01 00:00:00 EDT 2018

m⁶A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer.

Nature cell biology

Liu J,Eckert MA,Harada BT,Liu SM,Lu Z,Yu K,Tienda SM,Chryplewicz A,Zhu AC,Yang Y,Huang JT,Chen SM,Xu ZG,Leng XH,Yu XC,Cao J,Zhang Z,Liu J,Lengyel E,He C

Sat Sep 01 00:00:00 EDT 2018

The Role of m⁶A/m-RNA Methylation in Stress Response Regulation.

Neuron

Engel M,Eggert C,Kaplick PM,Eder M,Röh S,Tietze L,Namendorf C,Arloth J,Weber P,Rex-Haffner M,Geula S,Jakovcevski M,Hanna JH,Leshkowitz D,Uhr M,Wotjak CT,Schmidt MV,Deussing JM,Binder EB,Chen A

Wed Jul 25 00:00:00 EDT 2018

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Nucleic acids research

Du Y,Hou G,Zhang H,Dou J,He J,Guo Y,Li L,Chen R,Wang Y,Deng R,Huang J,Jiang B,Xu M,Cheng J,Chen GQ,Zhao X,Yu J

Fri Jun 01 00:00:00 EDT 2018

SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.

Nucleic acids research

Du Y,Hou G,Zhang H,Dou J,He J,Guo Y,Li L,Chen R,Wang Y,Deng R,Huang J,Jiang B,Xu M,Cheng J,Chen GQ,Zhao X,Yu J

Fri Jun 01 00:00:00 EDT 2018

METTL3 regulates alternative splicing of MyD88 upon the lipopolysaccharide-induced inflammatory response in human dental pulp cells.

Journal of cellular and molecular medicine

Feng Z,Li Q,Meng R,Yi B,Xu Q

Tue May 01 00:00:00 EDT 2018

Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m⁶A machinery component Wtap/Fl(2)d.

Genes & development

Knuckles P,Lence T,Haussmann IU,Jacob D,Kreim N,Carl SH,Masiello I,Hares T,Villaseñor R,Hess D,Andrade-Navarro MA,Biggiogera M,Helm M,Soller M,Bühler M,Roignant JY

Thu Mar 01 00:00:00 EST 2018

Dynamic m6A modification regulates local translation of mRNA in axons.

Nucleic acids research

Yu J,Chen M,Huang H,Zhu J,Song H,Zhu J,Park J,Ji SJ

Fri Feb 16 00:00:00 EST 2018

N⁶-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Molecular cell

Zhou J,Wan J,Shu XE,Mao Y,Liu XM,Yuan X,Zhang X,Hess ME,Brüning JC,Qian SB

Thu Feb 15 00:00:00 EST 2018

The epitranscriptome m6A writer METTL3 promotes chemo- and radioresistance in pancreatic cancer cells.

International journal of oncology

Taketo K,Konno M,Asai A,Koseki J,Toratani M,Satoh T,Doki Y,Mori M,Ishii H,Ogawa K

Thu Feb 01 00:00:00 EST 2018

Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.

Genetics

Xia H,Zhong C,Wu X,Chen J,Tao B,Xia X,Shi M,Zhu Z,Trudeau VL,Hu W

Thu Feb 01 00:00:00 EST 2018

Mettl3 Mutation Disrupts Gamete Maturation and Reduces Fertility in Zebrafish.

Genetics

Xia H,Zhong C,Wu X,Chen J,Tao B,Xia X,Shi M,Zhu Z,Trudeau VL,Hu W

Thu Feb 01 00:00:00 EST 2018

Thiol-linked alkylation of RNA to assess expression dynamics.

Nature methods

Herzog VA,Reichholf B,Neumann T,Rescheneder P,Bhat P,Burkard TR,Wlotzka W,von Haeseler A,Zuber J,Ameres SL

Fri Dec 01 00:00:00 EST 2017

m⁶A Facilitates eIF4F-Independent mRNA Translation.

Molecular cell

Coots RA,Liu XM,Mao Y,Dong L,Zhou J,Wan J,Zhang X,Qian SB

Thu Nov 02 00:00:00 EDT 2017

m⁶A Facilitates eIF4F-Independent mRNA Translation.

Molecular cell

Coots RA,Liu XM,Mao Y,Dong L,Zhou J,Wan J,Zhang X,Qian SB

Thu Nov 02 00:00:00 EDT 2017

The N⁶-methyladenosine (m⁶A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells.

Nature medicine

Vu LP,Pickering BF,Cheng Y,Zaccara S,Nguyen D,Minuesa G,Chou T,Chow A,Saletore Y,MacKay M,Schulman J,Famulare C,Patel M,Klimek VM,Garrett-Bakelman FE,Melnick A,Carroll M,Mason CE,Jaffrey SR,Kharas MG

Wed Nov 01 00:00:00 EDT 2017

Region-specific RNA m⁶A methylation represents a new layer of control in the gene regulatory network in the mouse brain.

Open biology

Chang M,Lv H,Zhang W,Ma C,He X,Zhao S,Zhang ZW,Zeng YX,Song S,Niu Y,Tong WM

Fri Sep 01 00:00:00 EDT 2017

The requirement of Mettl3-promoted MyoD mRNA maintenance in proliferative myoblasts for skeletal muscle differentiation.

Open biology

Kudou K,Komatsu T,Nogami J,Maehara K,Harada A,Saeki H,Oki E,Maehara Y,Ohkawa Y

Fri Sep 01 00:00:00 EDT 2017

NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation.

Journal of cellular biochemistry

Li Q,Li X,Tang H,Jiang B,Dou Y,Gorospe M,Wang W

Fri Sep 01 00:00:00 EDT 2017

RNA fate determination through cotranscriptional adenosine methylation and microprocessor binding.

Nature structural & molecular biology

Knuckles P,Carl SH,Musheev M,Niehrs C,Wenger A,Bühler M

Sat Jul 01 00:00:00 EDT 2017

N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein.

Nucleic acids research

Liu N,Zhou KI,Parisien M,Dai Q,Diatchenko L,Pan T

Fri Jun 02 00:00:00 EDT 2017

Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation.

Cell

Slobodin B,Han R,Calderone V,Vrielink JAFO,Loayza-Puch F,Elkon R,Agami R

Thu Apr 06 00:00:00 EDT 2017

m⁶A RNA Methylation Regulates the Self-Renewal and Tumorigenesis of Glioblastoma Stem Cells.

Cell reports

Cui Q,Shi H,Ye P,Li L,Qu Q,Sun G,Sun G,Lu Z,Huang Y,Yang CG,Riggs AD,He C,Shi Y

Tue Mar 14 00:00:00 EDT 2017

AMPK regulates lipid accumulation in skeletal muscle cells through FTO-dependent demethylation of N⁶-methyladenosine.

Scientific reports

Wu W,Feng J,Jiang D,Zhou X,Jiang Q,Cai M,Wang X,Shan T,Wang Y

Wed Feb 08 00:00:00 EST 2017

N(6)-methyladenosine of HIV-1 RNA regulates viral infection and HIV-1 Gag protein expression.

eLife

Tirumuru N,Zhao BS,Lu W,Lu Z,He C,Wu L

Sat Jul 02 00:00:00 EDT 2016

The m(6)A Methyltransferase METTL3 Promotes Translation in Human Cancer Cells.

Molecular cell

Lin S,Choe J,Du P,Triboulet R,Gregory RI

Thu May 05 00:00:00 EDT 2016

m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency.

Cell stem cell

Chen T,Hao YJ,Zhang Y,Li MM,Wang M,Han W,Wu Y,Lv Y,Hao J,Wang L,Li A,Yang Y,Jin KX,Zhao X,Li Y,Ping XL,Lai WY,Wu LG,Jiang G,Wang HL,Sang L,Wang XJ,Yang YG,Zhou Q

Thu Mar 05 00:00:00 EST 2015

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Science (New York, N.Y.)

Geula S,Moshitch-Moshkovitz S,Dominissini D,Mansour AA,Kol N,Salmon-Divon M,Hershkovitz V,Peer E,Mor N,Manor YS,Ben-Haim MS,Eyal E,Yunger S,Pinto Y,Jaitin DA,Viukov S,Rais Y,Krupalnik V,Chomsky E,Zerbib M,Maza I,Rechavi Y,Massarwa R,Hanna S,Amit I,Levanon EY,Amariglio N,Stern-Ginossar N,Novershtern N,Rechavi G,Hanna JH

Fri Feb 27 00:00:00 EST 2015

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.

Science (New York, N.Y.)

Fri Feb 27 00:00:00 EST 2015

m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells.

Cell stem cell

Batista PJ,Molinie B,Wang J,Qu K,Zhang J,Li L,Bouley DM,Lujan E,Haddad B,Daneshvar K,Carter AC,Flynn RA,Zhou C,Lim KS,Dedon P,Wernig M,Mullen AC,Xing Y,Giallourakis CC,Chang HY

Thu Dec 04 00:00:00 EST 2014

FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis.

Cell research

Zhao X,Yang Y,Sun BF,Shi Y,Yang X,Xiao W,Hao YJ,Ping XL,Chen YS,Wang WJ,Jin KX,Wang X,Huang CM,Fu Y,Ge XM,Song SH,Jeong HS,Yanagisawa H,Niu Y,Jia GF,Wu W,Tong WM,Okamoto A,He C,Rendtlew Danielsen JM,Wang XJ,Yang YG

Mon Dec 01 00:00:00 EST 2014

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation.

Nature chemical biology

Liu J,Yue Y,Han D,Wang X,Fu Y,Zhang L,Jia G,Yu M,Lu Z,Deng X,Dai Q,Chen W,He C

Sat Feb 01 00:00:00 EST 2014

N6-methyladenosine-dependent regulation of messenger RNA stability.

Nature

Wang X,Lu Z,Gomez A,Hon GC,Yue Y,Han D,Fu Y,Parisien M,Dai Q,Jia G,Ren B,Pan T,He C

Thu Jan 02 00:00:00 EST 2014

RNA-methylation-dependent RNA processing controls the speed of the circadian clock.

Cell

Fustin JM,Doi M,Yamaguchi Y,Hida H,Nishimura S,Yoshida M,Isagawa T,Morioka MS,Kakeya H,Manabe I,Okamura H

Thu Nov 07 00:00:00 EST 2013

Bioinformatics

Protein Aliases: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; m6a methyltransferase; methyltransferase-like 3; Methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase subunit METTL3

Gene Aliases: 2310024F18Rik; IME4; M6A; METTL3; MT-A70; MTA70; Spo8

UniProt ID: (Human) Q86U44, (Mouse) Q8C3P7

Entrez Gene ID: (Human) 56339, (Mouse) 56335, (Rat) 361035

Molecular Function: RNA metabolism protein RNA methyltransferase nucleic acid metabolism protein