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METTL5 Polyclonal Antibody

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Antibody Testing Data (9)

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METTL5 Antibody (16791-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 16791-1-AP) METTL5 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Image Gallery

METTL5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using METTL5 antibody 16791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

METTL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16791-1-AP (METTL5 antibody) at dilution of 1:100 (under 40x lens).

METTL5 Antibody in Immunoprecipitation (IP) — IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug.

Product Details

16791-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

METTL5 Fusion Protein Ag10232 (1-205 aa encoded by BC000921)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MKKVRLKEL ESRLQQVDGF EKPKLLLEQY PTRPHIAACM LYTIHNTYDD IENKVVADLG CGCGVLSIGT AMLGAGLCVG FDIDEDALEI FNRNAEEFEL TNIDMVQCDV CLLSNRMSKS FDTVIMNPPF GTKNNKGTDM AFLKTALEMA RTAVYSLHKS STREHVQKKA AEWKIKIDII AELRYDLPAS YKFHKKKSVD IEVDLI (1-205 aa encoded by B C000921)

Target Information

Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

N⁶-methyladenosine modification in 18S rRNA promotes tumorigenesis and chemoresistance via HSF4b/HSP90B1/mutant p53 axis.

Cell chemical biology

Chen B,Huang Y,He S,Yu P,Wu L,Peng H

Thu Feb 16 00:00:00 EST 2023

METTL3 regulates breast cancer-associated alternative splicing switches.

Oncogene

Achour C,Bhattarai DP,Groza P,Román ÁC,Aguilo F

Wed Mar 01 00:00:00 EST 2023

iScience

Zhang J,Chen J,Lu Y,Yang Y,Chen W,Shen B,Hu J,Jia P,Xu S,Shi Y,Ning Y,Wang J,Fang Y,Zhao S,Li Y,Dai Y,Zhang X,Xiang M,Tian Y,Liu Z,Song N,Ding X

Thu Dec 22 00:00:00 EST 2022

N⁶-methyladenosine (m⁶A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation.

Nature metabolism

Peng H,Chen B,Wei W,Guo S,Han H,Yang C,Ma J,Wang L,Peng S,Kuang M,Lin S

Mon Aug 01 00:00:00 EDT 2022

The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.

Bioengineered

Liu X,Ma H,Ma L,Li K,Kang Y

Tue Mar 01 00:00:00 EST 2022

The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma.

Bioengineered

Liu X,Ma H,Ma L,Li K,Kang Y

Tue Mar 01 00:00:00 EST 2022

Long noncoding RNA UBA6-AS1 inhibits the malignancy of ovarian cancer cells via suppressing the decay of UBA6 mRNA.

Bioengineered

Wang Y,Chen Z

Sat Jan 01 00:00:00 EST 2022

Loss of m⁶A Methyltransferase METTL5 Promotes Cardiac Hypertrophy Through Epitranscriptomic Control of SUZ12 Expression.

Frontiers in cardiovascular medicine

Han Y,Du T,Guo S,Wang L,Dai G,Long T,Xu T,Zhuang X,Liu C,Li S,Zhang D,Liao X,Dong Y,Lui KO,Tan X,Lin S,Chen Y,Huang ZP

Sat Mar 19 00:00:00 EDT 2022

The METTL5-TRMT112 N⁶-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation.

The Journal of biological chemistry

Sepich-Poore C,Zheng Z,Schmitt E,Wen K,Zhang ZS,Cui XL,Dai Q,Zhu AC,Zhang L,Sanchez Castillo A,Tan H,Peng J,Zhuang X,He C,Nachtergaele S

Tue Mar 01 00:00:00 EST 2022

The 18S rRNA m⁶ A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.

EMBO reports

Xing M,Liu Q,Mao C,Zeng H,Zhang X,Zhao S,Chen L,Liu M,Shen B,Guo X,Ma H,Chen H,Zhang J

Mon Oct 05 00:00:00 EDT 2020

Ribosome 18S m⁶A Methyltransferase METTL5 Promotes Translation Initiation and Breast Cancer Cell Growth.

Cell reports

Rong B,Zhang Q,Wan J,Xing S,Dai R,Li Y,Cai J,Xie J,Song Y,Chen J,Zhang L,Yan G,Zhang W,Gao H,Han JJ,Qu Q,Ma H,Tian Y,Lan F

Tue Dec 22 00:00:00 EST 2020

Bioinformatics

Protein Aliases: Methyltransferase-like protein 5; rRNA N6-adenosine-methyltransferase METTL5

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Gene Aliases: 2810410A08Rik; DC3; HSPC133; METTL5; RGD1566062

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UniProt ID: (Human) Q9NRN9, (Mouse) Q8K1A0

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Entrez Gene ID: (Human) 29081, (Mouse) 75422, (Rat) 502632

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Function(s)

methylation biological_process

Process(es)

Performance Guarantee

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METTL5 Polyclonal Antibody

METTL5 Antibody (16791-1-AP) in WB

Product Details

16791-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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