Proteintech

METTL5 Polyclonal Antibody

View all (6) METL5 antibodies

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METTL5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using METTL5 antibody 16791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

METTL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16791-1-AP (METTL5 antibody) at dilution of 1:100 (under 40x lens).

METTL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 16791-1-AP (METTL5 antibody) at dilution of 1:100 (under 10x lens).

METTL5 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

METTL5 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

METTL5 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 16791-1-AP (METTL5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

METTL5 Antibody in Immunoprecipitation (IP) — IP result of anti-METTL5 (IP:16791-1-AP, 4ug; Detection:16791-1-AP 1:500) with PC-3 cells lysate 3840 ug.

Product Details

16791-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	METTL5 Fusion Protein Ag10232 (1-205 aa encoded by BC000921) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.15 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MKKVRLKEL ESRLQQVDGF EKPKLLLEQY PTRPHIAACM LYTIHNTYDD IENKVVADLG CGCGVLSIGT AMLGAGLCVG FDIDEDALEI FNRNAEEFEL TNIDMVQCDV CLLSNRMSKS FDTVIMNPPF GTKNNKGTDM AFLKTALEMA RTAVYSLHKS STREHVQKKA AEWKIKIDII AELRYDLPAS YKFHKKKSVD IEVDLI (1-205 aa encoded by B C000921)

Target Information

Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Methyltransferase-like protein 5; rRNA N6-adenosine-methyltransferase METTL5

Gene Aliases: 2810410A08Rik; DC3; HSPC133; METTL5; RGD1566062

UniProt ID: (Human) Q9NRN9, (Mouse) Q8K1A0

Entrez Gene ID: (Human) 29081, (Mouse) 75422, (Rat) 502632

methylation biological_process

Process(es)

nucleic acid binding methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity