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Invitrogen
CD4 Monoclonal Antibody (S3.5), Pacific Orange™
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Product Details
MHCD0430
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
S3.5
Immunogen
Human CD4
Conjugate
Excitation/Emission Max
400/551 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with BSA
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
Analyte Specific Reagent
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T4 surface glycoprotein precursor; unnamed protein product
Gene Aliases: CD4; CD4mut; IMD79; Leu-3; OKT4D; T4
UniProt ID: (Human) P01730
Entrez Gene ID: (Human) 920
virus receptor activity
transmembrane signaling receptor activity
receptor binding
extracellular matrix structural constituent
protein binding
zinc ion binding
lipid binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein complex binding
signaling receptor activity
interleukin-16 binding
interleukin-16 receptor activity
MHC class II protein binding
identical protein binding
protein homodimerization activity
protein tyrosine kinase binding
positive regulation of protein phosphorylation
adaptive immune response
immune system process
positive regulation of immune system process
immune response
cell adhesion
signal transduction
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of calcium ion transport into cytosol
calcium-mediated signaling
T cell differentiation
macrophage differentiation
response to estradiol
maintenance of protein location in cell
positive regulation of interleukin-2 production
response to vitamin D
positive regulation of kinase activity
helper T cell enhancement of adaptive immune response
interleukin-15-mediated signaling pathway
positive regulation of T cell proliferation
T cell activation
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
T cell selection
response to ethanol
positive regulation of monocyte differentiation
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of viral entry into host cell
viral entry into host cell
defense response to Gram-negative bacterium
positive regulation of calcium-mediated signaling
regulation of T cell activation
positive regulation of T cell activation
positive regulation of multicellular organismal process
regulation of calcium ion transport
positive regulation of ERK1 and ERK2 cascade
cellular response to granulocyte macrophage colony-stimulating factor stimulus
response to methamphetamine hydrochloride
cellular response to ionomycin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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