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Invitrogen
CD45RA Monoclonal Antibody (MEM-56), PE-Texas Red
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Product Details
MHCD45RA17
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
MEM-56
Immunogen
Human CD45RA
Conjugate
PE-Texas Red
PE-Texas Red
PE-Texas Red
Excitation/Emission Max
567/615 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS with BSA, 10% sucrose
Contains
0.1% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The R-phycoerythrin (PE)-Texas Red conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
Target Information
CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.
Analyte Specific Reagent
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD45 antigen; glycoprotein 180; Ly-5; Lyt-4; protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, c polypeptide; T100; T200 glycoprotein; T200 leukocyte common antigen; unnamed protein product
Gene Aliases: B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200
UniProt ID: (Human) P08575
Entrez Gene ID: (Human) 5788
negative regulation of transcription from RNA polymerase II promoter
natural killer cell differentiation
negative regulation of T cell mediated cytotoxicity
negative regulation of cytokine-mediated signaling pathway
hematopoietic progenitor cell differentiation
positive regulation of immunoglobulin production
negative regulation of protein kinase activity
protein dephosphorylation
negative regulation of cell adhesion involved in substrate-bound cell migration
signal transduction
cell surface receptor signaling pathway
response to gamma radiation
regulation of gene expression
dephosphorylation
B cell differentiation
T cell differentiation
positive regulation of B cell proliferation
regulation of interleukin-8 production
negative regulation of interleukin-2 production
positive regulation of interleukin-2 production
positive regulation of tumor necrosis factor production
B cell proliferation
positive regulation of T cell proliferation
T cell activation
positive regulation of MAPK cascade
cell cycle phase transition
plasma membrane raft distribution
positive regulation of protein kinase activity
negative regulation of JAK-STAT cascade
bone marrow development
stem cell development
regulation of phagocytosis
positive regulation of phagocytosis
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of T cell receptor signaling pathway
positive regulation of antigen receptor-mediated signaling pathway
release of sequestered calcium ion into cytosol
defense response to virus
regulation of cell cycle
positive regulation of Fc receptor mediated stimulatory signaling pathway
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
negative regulation of interleukin-4-mediated signaling pathway
negative regulation of microglial cell activation
response to aldosterone
DN2 thymocyte differentiation
positive regulation of hematopoietic stem cell migration
positive regulation of stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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