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          • Primary Antibodies ›
          • MICAL1 Antibodies

          Invitrogen

          MICAL1 Polyclonal Antibody

          View all (32) MICAL1 antibodies

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          Cite MICAL1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          MICAL1 Antibody in Western Blot (WB)
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          MICAL1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          MICAL1 Antibody (PA5-90518) in WB

          Western blot analysis of MICAL1 in extracts of various cell lines. Samples were incubated with MICAL1 Polyclonal antibody (Product # PA5-90518) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          MICAL1 Antibody in Western Blot (WB)

          Product Details

          PA5-90518

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein (or fragment)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.75 mg/mL

          Amount

          175 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2806106

          Product Specific Information

          Positive Samples: A-549, HT-29, Raji; Cellular Location: Cytoplasm, cytoskeleton

          Target Information

          May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: [F-actin]-methionine sulfoxide oxidase MICAL1; [F-actin]-monooxygenase MICAL1; CasL interacting molecule MICAL; MICAL-1; microtubule associated monoxygenase, calponin and LIM domain containing 1; mMical1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1; unnamed protein product

          View more View less

          Gene Aliases: MICAL; MICAL-1; MICAL1; NICAL

          View more View less

          UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3

          View more View less

          Entrez Gene ID: (Human) 64780, (Mouse) 171580

          View more View less

          Function(s)
          actin binding monooxygenase activity protein binding NAD(P)H oxidase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen SH3 domain binding protein kinase binding small GTPase binding metal ion binding actin filament binding FAD binding F-actin monooxygenase activity zinc ion binding 3-(3-hydroxyphenyl)propionate hydroxylase activity Rab GTPase binding 4-chlorobenzaldehyde oxidase activity 3,5-xylenol methylhydroxylase activity phenylacetate hydroxylase activity 4-nitrophenol 4-monooxygenase activity dimethyl sulfide monooxygenase activity alpha-pinene monooxygenase [NADH] activity phenanthrene 9,10-monooxygenase activity 1-hydroxy-2-naphthoate hydroxylase activity toluene 4-monooxygenase activity xylene monooxygenase activity dibenzothiophene monooxygenase activity 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity chlorophenol 4-monooxygenase activity carbon disulfide oxygenase activity toluene 2-monooxygenase activity 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity phenanthrene 1,2-monooxygenase activity tetrahydrofuran hydroxylase activity styrene monooxygenase activity toluene-4-sulfonate monooxygenase activity toluene-sulfonate methyl-monooxygenase activity 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity 2-hydroxy-phenylacetate hydroxylase activity 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity phenanthrene 3,4-monooxygenase activity toluene 3-monooxygenase activity 4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity limonene monooxygenase activity 2-methylnaphthalene hydroxylase activity 1-methylnaphthalene hydroxylase activity bisphenol A hydroxylase A activity salicylate 5-hydroxylase activity isobutylamine N-hydroxylase activity branched-chain dodecylbenzene sulfonate monooxygenase activity 3-HSA hydroxylase activity 4-hydroxypyridine-3-hydroxylase activity 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity 6-hydroxynicotinate 3-monooxygenase activity tocotrienol omega-hydroxylase activity thalianol hydroxylase activity
          Process(es)
          negative regulation of protein phosphorylation cytoskeleton organization sulfur oxidation actin filament depolymerization negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process signal transduction actin filament bundle assembly regulation of regulated secretory pathway
          It has to be done as per old AB suggested Products section.

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