Invitrogen

MID1 Polyclonal Antibody

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MID1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of MID1 in paraffin-embedded human tonsil carcinoma tissue. Samples were incubated with MID1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:50.

MID1 Antibody in Western Blot (WB) — Western blot analysis of MID1 in Lane 1: A549 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: 786-O whole cell lysate (40 µg), Lane 4: L02 whole cell lysate (40 µg), Lane 5: HepG2 whole cell lysate (40 µg). Samples were incubated with MID1 polyclonal antibody (Product # PA5-36305) at a dilution of 1:500.

MID1 Antibody in Immunoprecipitation (IP) — Figure 5 TRIM18 interacts with protein tyrosine phosphatases (PTP1B) and promotes PTP1B ubiquitination. (A) HK-2 cells were treated with HG and transduced with indicated vectors, and then TRIM18, SHP-1, SOCS1, PTP1B, and PIAS1 expression was measured. (B,C) Cell lysates were immunoprecipitated and then blotted with the indicated antibodies. (D-F) Cells were transduced with indicated vectors with/without 10 muM MG132, and then PTP1B expression was measured. (G) PTP1B was immunoprecipitated and immunoblotted. Values are presented as mean +- SD. n = 3/group. *** p < 0.001 vs. shNC or vector+vehicle. ### p < 0.001 vs. oeTRIM18+vehicle.

Product Details

PA5-36305

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to amino acids 71-120 of human Midline-1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2553463

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 75 kDa.

Purity is >95% by SDS-PAGE.

Target Information

Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease.

Frontiers in physiology

Chen Q,Gao C,Wang M,Fei X,Zhao N

Published figure using MID1 polyclonal antibody (Product # PA5-36305) in Immunoprecipitation

Fri Aug 27 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase Midline-1; Finger on X and Y (in rat only on X); Midin; midline 1 (Opitz/BBB syndrome); midline 1 RING finger protein; midline-1; Opitz/BBB syndrome; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; tripartite motif protein TRIM18; Tripartite motif-containing protein 18; zinc finger on X and Y, mouse, homolog of

Gene Aliases: 61B3-R; BBBG1; DXHXS1141; FXY; GBBB1; MID1; MIDIN; Midline1; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY

UniProt ID: (Human) O15344, (Rat) P82458, (Mouse) O70583

Entrez Gene ID: (Human) 4281, (Rat) 54252, (Mouse) 17318

Molecular Function: ubiquitin-protein ligase