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Invitrogen
MID1 Polyclonal Antibody
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Product Details
PA5-36305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 71-120 of human Midline-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 75 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: contains ring finger domain; E3 ubiquitin-protein ligase Midline-1; Finger on X and Y (in rat only on X); Midin; midline 1 (Opitz/BBB syndrome); midline 1 RING finger protein; midline-1; Opitz/BBB syndrome; Putative transcription factor XPRF; RBCC protein; RING finger protein 59; RING finger protein Midline-1; RING-type E3 ubiquitin transferase Midline-1; tripartite motif protein TRIM18; Tripartite motif-containing protein 18; unnamed protein product; zinc finger on X and Y, mouse, homolog of
Gene Aliases: 61B3-R; BBBG1; DXHXS1141; FXY; GBBB; GBBB1; MID1; MIDIN; Midline1; OGS1; OS; OSX; RNF59; TRIM18; XPRF; ZNFXY
UniProt ID: (Human) O15344, (Rat) P82458, (Mouse) O70583
Entrez Gene ID: (Human) 4281, (Rat) 54252, (Mouse) 17318
protein binding
microtubule binding
zinc ion binding
transferase activity
enzyme binding
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
metal ion binding
phosphoprotein binding
ubiquitin protein ligase activity
ligase activity
protein heterodimerization activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
microtubule cytoskeleton organization
negative regulation of microtubule depolymerization
pattern specification process
positive regulation of stress-activated MAPK cascade
protein localization to microtubule
regulation of microtubule cytoskeleton organization
positive regulation of intracellular signal transduction
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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