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Invitrogen
MIDN Polyclonal Antibody
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FIGURE: 1 / 1
MIDN Antibody (PA5-50521) in WB
Western Blot analysis of MIDN was performed using Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Stomach tissue lysates Lane 3: Rat Cerebrum tissue lysates. The membrane was probed with MIDN Polyclonal Antibody (Product # PA5-50521) at 1:1,000 dilution. Secondary antibody: at 1:20,000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa.
Product Details
PA5-50521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues near the N terminal of human Midnolin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total MIDN protein.
Target Information
Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp547M072; Midbrain nucleolar protein; Midnolin
Gene Aliases: 3000003C15Rik; MIDN
UniProt ID: (Human) Q504T8, (Rat) D4AE48, (Mouse) Q3TPJ7
Entrez Gene ID: (Human) 90007, (Rat) 314623, (Mouse) 59090
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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