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Product Details
PA5-59825
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.04-0.4 µg/mL | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human MIDN |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2643967 |
Product Specific Information
Immunogen sequence: VPKERLALLH KDTRLSSGKL QEFGVGDGSK LTLVPTVEAG LMSQASRPEQ SVMQALESLT ETQVSDFLSG RSPLTLALRV GDHMMFVQLQ LAAQHAPLQ
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 70%.
Target Information
Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp547M072; Midbrain nucleolar protein; Midnolin
Gene Aliases: MIDN
UniProt ID: (Human) Q504T8
Entrez Gene ID: (Human) 90007
Performance Guarantee
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