Invitrogen

MIF Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (45) MIF antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

MIF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Macrophage migration inhibitory factor was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MIF Polyclonal Antibody (Product # PA5-89525) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and secreted localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MIF Antibody in Western Blot (WB) — Western Blot was performed using Anti-MIF Polyclonal Antibody (Product # PA5-89525) and a 15 kDa band corresponding to Macrophage migration inhibitory factor was observed across cell lines tested and was upregulated in U-87 MG upon VEGF treatment. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with PTI (1X, 4hrs) (Lane 2), U-937 (Lane 3), U-87 MG (Lane 4), U-87 MG treated with Rec. hVEGF (100 ng/mL, 48hrs) (Lane 5), Jurkat (Lane 6), HUVEC (Lane 7), RAW 264.7 (Lane 8), HEK-293 (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MIF Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using MIF Polyclonal Antibody (Product # PA5-89525), shows increase in expression of MIF protein upon VEGF treatment in U-87 MG cell line.

Product Details

PA5-89525

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIF

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

1.07 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2805629

Product Specific Information

Immunogen sequence: MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA; Positive Samples: HL-60, THP-1, SW620, Mouse kidney, Mouse lung, Mouse heart; Cellular Location: Cytoplasm

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

Gene Aliases: GIF; GLIF; MIF; MMIF

UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

Molecular Function: decarboxylase