Invitrogen

MIF Polyclonal Antibody

View all (11) MIF antibodies

Create Citation

Create Citation

Product Image Gallery

MIF Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of A-549 cells using MIF Polyclonal antibody (Product # PA5-89525). Blue: DAPI for nuclear staining.

MIF Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using MIF Polyclonal antibody (Product # PA5-89525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

Tested Applications

Dilution

Immunofluorescence (IF)

1:10-1:100

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-115 of human MIF

Conjugate

Unconjugated

Form

liquid

Concentration

0.87 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MPMFIVNTNV PRASVPDGFL RVNFVDPAAS SSAEDSLSDA AGVGVDGPNV SFQNGGDTVL SEGSSLHSGG GGGSGHHQHY YYDTHTNTYY LRTFGHNTMD AVPRIDHYRH TAAQLGEKLL RPSLAELHDE LEKEPFEDGF ANGEESTPTR DAVVTYTAES K; Positive Samples: HL-60, THP-1, SW620, Mouse kidney, Mouse lung, Mouse heart; Cellular Location: Cytoplasm

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MIF; Phenylpyruvate tautomerase

Gene Aliases: GIF; GLIF; MIF; MMIF

UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

prostaglandin biosynthetic process negative regulation of mature B cell apoptotic process inflammatory response cell surface receptor signaling pathway cell aging cell proliferation negative regulation of gene expression positive regulation of protein kinase A signaling carboxylic acid metabolic process DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of cellular protein metabolic process negative regulation of myeloid cell apoptotic process positive regulation of peptidyl-serine phosphorylation positive regulation of phosphorylation regulation of macrophage activation negative regulation of apoptotic process positive regulation of MAP kinase activity negative regulation of DNA damage response, signal transduction by p53 class mediator innate immune response positive regulation of fibroblast proliferation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive chemotaxis positive regulation of prostaglandin secretion involved in immune response positive regulation of myeloid leukocyte cytokine production involved in immune response protein homotrimerization positive regulation of ERK1 and ERK2 cascade negative regulation of cell cycle arrest positive regulation of arachidonic acid secretion negative regulation of cell aging negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of protein phosphorylation brain renin-angiotensin system immune system process positive regulation of acute inflammatory response positive regulation of adaptive immune response spermatogenesis positive regulation of smooth muscle cell migration regulation of cell proliferation drinking behavior positive regulation of potassium ion transport positive regulation of glycolytic process positive regulation of glucose import positive regulation of axon regeneration positive regulation of immune response hair follicle development brain development aging metabolic process response to heat response to mechanical stimulus response to hormone response to inorganic substance response to estradiol response to lipopolysaccharide response to progesterone response to menaquinone response to insulin response to vitamin E wound healing response to drug skin development response to glucocorticoid cellular response to hydrogen peroxide cellular response to hypoxia

Process(es)

dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity isomerase activity protein complex binding

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (587)
Alexa Fluor® Anti-Rabbit Secondary Antibodies (130)
HRP Anti-Rabbit Secondary Antibodies (80)