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Product Details
710240
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7HCLC
Immunogen
Recombinant protein corresponding to amino acids 2-115 of human MIF
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with non-human primate, mouse and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MGC107654; MIF; migration inhibitory factor; Phenylpyruvate tautomerase; unnamed protein product
Gene Aliases: GIF; GLIF; MIF; MMIF
UniProt ID: (Human) P14174
Entrez Gene ID: (Human) 4282
prostaglandin biosynthetic process
positive regulation of cytokine production
immune system process
negative regulation of mature B cell apoptotic process
inflammatory response
cell surface receptor signaling pathway
positive regulation of cell proliferation
negative regulation of gene expression
negative regulation of macrophage chemotaxis
carboxylic acid metabolic process
DNA damage response, signal transduction by p53 class mediator
negative regulation of cell migration
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of tumor necrosis factor production
negative regulation of myeloid cell apoptotic process
regulation of cell proliferation
positive regulation of phosphorylation
regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
innate immune response
positive regulation of fibroblast proliferation
positive chemotaxis
negative regulation of protein metabolic process
positive regulation of prostaglandin secretion involved in immune response
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
regulation of cellular response to stress
positive regulation of arachidonic acid secretion
cellular senescence
positive regulation of cAMP/PKA signal transduction
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
negative regulation of cellular senescence
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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