Invitrogen

MIP-3 alpha Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 References

View all (26) CCL20 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

MIP-3 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of MIP-3 alpha in paraffin-embedded human-liver tissue using a MIP-3 alpha Polyclonal Antibody (Product # PA5-68465) at a 1:200 dilution. (Original magnification: x200).

MIP-3 alpha Antibody — Fig. 4 Pioglitazone treatment significantly reduces RGC loss, microgliosis, Muller cell activation, and CCL20 expression in the retina. a Representative immunofluorescent images showing RGC (H&E and RBPMS), Iba1 (red), GFAP (green), or CCL20 (bright field) immunoreactivity in the retina with or without PG treatment 7 days after rTBI. Scale bar 100 mum. Inset high magnification images of the boxed areas showing CCL20 expression in the retinal ganglionic cells, scale bar 20 mum. Blue fluorescence shows DAPI. Scale bar 100 mum. b - f Histograms showing average count of RGC ( b , c ), Iba1-positive cells ( d ), average fluorescence intensity of GFAP immunoreactivity ( e ), or CCL20 immunoreactivity ( f ) as quantitated by ImageJ. * p < 0.01, ** p < 0.001, *** p < 0.0001. {TM}

Product Details

PA5-68465

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthesized peptide derived from human MIP-3 alpha [aa 31-80]. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2688429

Product Specific Information

Predicted to react with Mouse samples.

Target Information

Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MAPK mutations and cigarette smoke promote the pathogenesis of pulmonary Langerhans cell histiocytosis.

JCI insight

Liu H,Osterburg AR,Flury J,Swank Z,McGraw DW,Gupta N,Wikenheiser-Brokamp KA,Kumar A,Tazi A,Inoue Y,Hirose M,McCormack FX,Borchers MT

PA5-68465 was used in Immunohistochemistry to establish a cigarette smoke-exposed (CS-exposed) BRAF-V600E-mutant mouse model that recapitulates many hallmark characteristics of PLCH.

Thu Feb 27 00:00:00 EST 2020

CCL20-CCR6 axis modulated traumatic brain injury-induced visual pathologies.

Journal of neuroinflammation

Das M,Tang X,Han JY,Mayilsamy K,Foran E,Biswal MR,Tzekov R,Mohapatra SS,Mohapatra S

PA5-68465 was used in Immunohistochemistry-immunofluorescence to look into how much CCL20-CCR6 axis plays a role in mediating rTBI-induced visual dysfunction.

Fri May 31 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: beta chemokine exodus-1; Beta-chemokine exodus-1; C-C motif chemokine 20; CC chemokine LARC; CC chemokine ST38; CCL20(1-64); CCL20(1-67); CCL20(2-70); chemokine (C-C motif) ligand 20; Liver and activation-regulated chemokine; M-MIP-3-alpha; Macrophage inflammatory protein 3 alpha; MIP 3 a; MIP 3 α; MIP 3alpha; MIP 3α; MIP-3-alpha; MIP3 a; MIP3 alpha; MIP3 α; small inducible cytokine subfamily A (Cys-Cys), member 20; small inducible cytokine subfamily A20; Small-inducible cytokine A20

Gene Aliases: CCL20; CKb4; Exodus; exodus-1; LARC; MIP-3-alpha; MIP-3[a]; MIP-3A; MIP3A; SCYA20; ST38

UniProt ID: (Human) P78556, (Mouse) O89093

Entrez Gene ID: (Human) 6364, (Mouse) 20297

Molecular Function: cytokine intercellular signal molecule