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Invitrogen
MIP-3 beta Polyclonal Antibody
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FIGURE: 1 / 4
MIP-3 beta Antibody (PA5-47958) in ICC/IF
Immunocytochemistry analysis of MIP-3 beta in immersion fixed mouse dendritic cells. Samples were incubated in MIP-3 beta polyclonal antibody (Product # PA5-47958) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Product Details
PA5-47958
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta Gly26-Val107 (Ser108LeuGlu)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
In direct ELISAs, less than 30% cross-reactivity with recombinant rat CCL19/MIP-3 beta is observed and less than 10% cross-reactivity with recombinant human CCL19/MIP-3 beta is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.
Target Information
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine; C-C motif chemokine 19; chemokine CCL19; EBI-1 ligand chemokine; EBI1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; M-MIP-3-beta; small inducible cytokine A19; Small-inducible cytokine A19
Gene Aliases: Ccl19; CKb11; ELC; exodus-3; MIP3B; Scya19
UniProt ID: (Mouse) O70460
Entrez Gene ID: (Mouse) 24047
establishment of T cell polarity
dendritic cell chemotaxis
myeloid dendritic cell chemotaxis
response to prostaglandin E
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of protein kinase activity
positive regulation of JNK cascade
release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
response to nitric oxide
positive regulation of neutrophil chemotaxis
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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