Proteintech

MKS1 Polyclonal Antibody

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MKS1 Antibody in Immunocytochemistry (ICC/IF) — IF result from Dr. Corbit, Kevin. anti-MKS1 (16206-1-AP) marks the transition zone of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

MKS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer using 16206-1-AP (MKS1 antibody) at dilution of 1:50 (under 40x lens).

MKS1 Antibody in Western Blot (WB) — Mouse uterus tissue were subjected to SDS PAGE followed by western blot with 16206-1-AP (MKS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MKS1 Antibody in Immunoprecipitation (IP) — IP result of anti-MKS1 (IP:16206-1-AP, 3ug; Detection:16206-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

Product Details

16206-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 20 publications 20 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MKS1 Fusion Protein Ag9177 (1-321 aa encoded by BC010061)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MTTAASEVP SFLVERMANV RRRRQDRRGM EGGILKSRIV TWEPSEEFVR NNHVINTPLQ TMHIMADLGP YKKLGYKKYE HVLCTLKVDS NGVITVKPDF TGLKGPYRIE TEGEKQELWK YTIDNVSPHA QPEEEERERR VFKDLYGRHK EYLSSLVGTD FEMTVPGALR LFVNGEVVSA QGYEYDNLYV HFFVELPTAH WSSPAFQQLS GVTQTCTTKS LAMDKVAHFS YPFTFEAFFL HEDESSDALP EWPVLYCEVL SLDFWQRYRV EGYGAVVLPA TPGSHTLTVS TWRPVELGTV AELRRFFIGG SLELEDLSYV RI (1-321 aa encoded by BC010061)

Target Information

The protein encoded by this gene localizes to the basal body and is required for formation of the primary cilium in ciliated epithelial cells. Mutations in this gene result in Meckel syndrome type 1 and in Bardet-Biedl syndrome type 13. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1.

eLife

Szymanska K,Boldt K,Logan CV,Adams M,Robinson PA,Ueffing M,Zeqiraj E,Wheway G,Johnson CA

Wed Feb 16 00:00:00 EST 2022

Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1.

eLife

Szymanska K,Boldt K,Logan CV,Adams M,Robinson PA,Ueffing M,Zeqiraj E,Wheway G,Johnson CA

Wed Feb 16 00:00:00 EST 2022

LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole.

The Journal of cell biology

Shen XL,Yuan JF,Qin XH,Song GP,Hu HB,Tu HQ,Song ZQ,Li PY,Xu YL,Li S,Jian XX,Li JN,He CY,Yu XP,Liang LY,Wu M,Han QY,Wang K,Li AL,Zhou T,Zhang YC,Wang N,Li HY

Mon Jan 03 00:00:00 EST 2022

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

eLife

Quidwai T,Wang J,Hall EA,Petriman NA,Leng W,Kiesel P,Wells JN,Murphy LC,Keighren MA,Marsh JA,Lorentzen E,Pigino G,Mill P

Thu Nov 04 00:00:00 EDT 2021

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Science advances

Chen C,Xu Q,Zhang Y,Davies BA,Huang Y,Katzmann DJ,Harris PC,Hu J,Ling K

Tue Jun 01 00:00:00 EDT 2021

ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

Developmental cell

Ran J,Liu M,Feng J,Li H,Ma H,Song T,Cao Y,Zhou P,Wu Y,Yang Y,Yang Y,Yu F,Guo H,Zhang L,Xie S,Li D,Gao J,Zhang X,Zhu X,Zhou J

Mon May 04 00:00:00 EDT 2020

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Nature communications

Tu HQ,Qin XH,Liu ZB,Song ZQ,Hu HB,Zhang YC,Chang Y,Wu M,Huang Y,Bai YF,Wang G,Han QY,Li AL,Zhou T,Liu F,Zhang XM,Li HY

Tue Dec 11 00:00:00 EST 2018

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Nature communications

Tu HQ,Qin XH,Liu ZB,Song ZQ,Hu HB,Zhang YC,Chang Y,Wu M,Huang Y,Bai YF,Wang G,Han QY,Li AL,Zhou T,Liu F,Zhang XM,Li HY

Tue Dec 11 00:00:00 EST 2018

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Biophysical journal

Weng RR,Yang TT,Huang CE,Chang CW,Wang WJ,Liao JC

Tue Jul 17 00:00:00 EDT 2018

Super-Resolution Imaging Reveals TCTN2 Depletion-Induced IFT88 Lumen Leakage and Ciliary Weakening.

Biophysical journal

Weng RR,Yang TT,Huang CE,Chang CW,Wang WJ,Liao JC

Tue Jul 17 00:00:00 EDT 2018

Cell type-specific regulation of ciliary transition zone assembly in vertebrates.

The EMBO journal

Wiegering A,Dildrop R,Kalfhues L,Spychala A,Kuschel S,Lier JM,Zobel T,Dahmen S,Leu T,Struchtrup A,Legendre F,Vesque C,Schneider-Maunoury S,Saunier S,Rüther U,Gerhardt C

Tue May 15 00:00:00 EDT 2018

Cell type-specific regulation of ciliary transition zone assembly in vertebrates.

The EMBO journal

Wiegering A,Dildrop R,Kalfhues L,Spychala A,Kuschel S,Lier JM,Zobel T,Dahmen S,Leu T,Struchtrup A,Legendre F,Vesque C,Schneider-Maunoury S,Saunier S,Rüther U,Gerhardt C

Tue May 15 00:00:00 EDT 2018

Glucose deprivation induces primary cilium formation through mTORC1 inactivation.

Journal of cell science

Takahashi K,Nagai T,Chiba S,Nakayama K,Mizuno K

Mon Jan 08 00:00:00 EST 2018

Glucose deprivation induces primary cilium formation through mTORC1 inactivation.

Journal of cell science

Takahashi K,Nagai T,Chiba S,Nakayama K,Mizuno K

Mon Jan 08 00:00:00 EST 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 EST 2018

The small GTPase RSG1 controls a final step in primary cilia initiation.

The Journal of cell biology

Agbu SO,Liang Y,Liu A,Anderson KV

Tue Jan 02 00:00:00 EST 2018

INPP5E regulates phosphoinositide-dependent cilia transition zone function.

The Journal of cell biology

Dyson JM,Conduit SE,Feeney SJ,Hakim S,DiTommaso T,Fulcher AJ,Sriratana A,Ramm G,Horan KA,Gurung R,Wicking C,Smyth I,Mitchell CA

Mon Jan 02 00:00:00 EST 2017

INPP5E regulates phosphoinositide-dependent cilia transition zone function.

The Journal of cell biology

Dyson JM,Conduit SE,Feeney SJ,Hakim S,DiTommaso T,Fulcher AJ,Sriratana A,Ramm G,Horan KA,Gurung R,Wicking C,Smyth I,Mitchell CA

Mon Jan 02 00:00:00 EST 2017

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.

Human molecular genetics

Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

Fri Jul 15 00:00:00 EDT 2016

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.

Human molecular genetics

Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

Fri Jul 15 00:00:00 EDT 2016

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.

Nature communications

Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R

Fri May 13 00:00:00 EDT 2016

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.

Nature communications

Fri May 13 00:00:00 EDT 2016

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Nature communications

Xu Q,Zhang Y,Wei Q,Huang Y,Hu J,Ling K

Fri Feb 26 00:00:00 EST 2016

Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.

Nature communications

Xu Q,Zhang Y,Wei Q,Huang Y,Hu J,Ling K

Fri Feb 26 00:00:00 EST 2016

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

Journal of medical genetics

Slaats GG,Isabella CR,Kroes HY,Dempsey JC,Gremmels H,Monroe GR,Phelps IG,Duran KJ,Adkins J,Kumar SA,Knutzen DM,Knoers NV,Mendelsohn NJ,Neubauer D,Mastroyianni SD,Vogt J,Worgan L,Karp N,Bowdin S,Glass IA,Parisi MA,Otto EA,Johnson CA,Hildebrandt F,van Haaften G,Giles RH,Doherty D

Fri Jan 01 00:00:00 EST 2016

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

Journal of medical genetics

Fri Jan 01 00:00:00 EST 2016

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

Journal of medical genetics

Fri Jan 01 00:00:00 EST 2016

MKS1 regulates ciliary INPP5E levels in Joubert syndrome.

Journal of medical genetics

Fri Jan 01 00:00:00 EST 2016

Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone.

Scientific reports

Yang TT,Su J,Wang WJ,Craige B,Witman GB,Tsou MF,Liao JC

Mon Sep 14 00:00:00 EDT 2015

Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone.

Scientific reports

Yang TT,Su J,Wang WJ,Craige B,Witman GB,Tsou MF,Liao JC

Mon Sep 14 00:00:00 EDT 2015

Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.

Scientific reports

Ran J,Yang Y,Li D,Liu M,Zhou J

Thu Aug 06 00:00:00 EDT 2015

Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly.

Scientific reports

Ran J,Yang Y,Li D,Liu M,Zhou J

Thu Aug 06 00:00:00 EDT 2015

TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.

The Journal of cell biology

Roberson EC,Dowdle WE,Ozanturk A,Garcia-Gonzalo FR,Li C,Halbritter J,Elkhartoufi N,Porath JD,Cope H,Ashley-Koch A,Gregory S,Thomas S,Sayer JA,Saunier S,Otto EA,Katsanis N,Davis EE,Attié-Bitach T,Hildebrandt F,Leroux MR,Reiter JF

Mon Apr 13 00:00:00 EDT 2015

TMEM231, mutated in orofaciodigital and Meckel syndromes, organizes the ciliary transition zone.

The Journal of cell biology

Mon Apr 13 00:00:00 EDT 2015

Lineage specificity of primary cilia in the mouse embryo.

Nature cell biology

Bangs FK,Schrode N,Hadjantonakis AK,Anderson KV

Sun Feb 01 00:00:00 EST 2015

Lineage specificity of primary cilia in the mouse embryo.

Nature cell biology

Bangs FK,Schrode N,Hadjantonakis AK,Anderson KV

Sun Feb 01 00:00:00 EST 2015

CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6.

Cell research

Yang Y,Ran J,Liu M,Li D,Li Y,Shi X,Meng D,Pan J,Ou G,Aneja R,Sun SC,Zhou J

Sat Nov 01 00:00:00 EDT 2014

CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6.

Cell research

Yang Y,Ran J,Liu M,Li D,Li Y,Shi X,Meng D,Pan J,Ou G,Aneja R,Sun SC,Zhou J

Sat Nov 01 00:00:00 EDT 2014

Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes.

PLoS genetics

Hall EA,Keighren M,Ford MJ,Davey T,Jarman AP,Smith LB,Jackson IJ,Mill P

Wed Aug 20 00:00:00 EDT 2014

Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes.

PLoS genetics

Hall EA,Keighren M,Ford MJ,Davey T,Jarman AP,Smith LB,Jackson IJ,Mill P

Wed Aug 20 00:00:00 EDT 2014

Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

Developmental dynamics : an official publication of the American Association of Anatomists

Nozawa YI,Yao E,Lin C,Yang JH,Wilson CW,Gacayan R,Chuang PT

Fri Nov 01 00:00:00 EDT 2013

Fused (Stk36) is a ciliary protein required for central pair assembly and motile cilia orientation in the mammalian oviduct.

Developmental dynamics : an official publication of the American Association of Anatomists

Nozawa YI,Yao E,Lin C,Yang JH,Wilson CW,Gacayan R,Chuang PT

Fri Nov 01 00:00:00 EDT 2013

Visualizing renal primary cilia.

Nephrology (Carlton, Vic.)

Deane JA,Verghese E,Martelotto LG,Cain JE,Galtseva A,Rosenblum ND,Watkins DN,Ricardo SD

Fri Mar 01 00:00:00 EST 2013

The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.

Cell

Goetz SC,Liem KF,Anderson KV

Fri Nov 09 00:00:00 EST 2012

The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis.

Cell

Goetz SC,Liem KF,Anderson KV

Fri Nov 09 00:00:00 EST 2012

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 EDT 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 EDT 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 EDT 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Meckel syndrome type 1 protein; Meckel syndrome type 1 protein homolog; POC12 centriolar protein homolog; Tectonic-like complex member MKS1

Gene Aliases: AK190930; B8d3; BBS13; MES; MKS; MKS1; POC12

UniProt ID: (Human) Q9NXB0, (Mouse) Q5SW45, (Rat) Q499Q5

Entrez Gene ID: (Human) 54903, (Mouse) 380718, (Rat) 287612

Molecular Function: structural protein

Function(s)

neural tube closure determination of left/right symmetry epithelial structure maintenance nonmotile primary cilium assembly cilium assembly embryonic digit morphogenesis embryonic skeletal system development branching morphogenesis of an epithelial tube inner ear receptor stereocilium organization cilium morphogenesis head development regulation of canonical Wnt signaling pathway common bile duct development regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning motile primary cilium assembly embryonic brain development regulation of Wnt signaling pathway, planar cell polarity pathway regulation of smoothened signaling pathway cell projection organization motile cilium assembly

Process(es)

protein binding

MKS1 Polyclonal Antibody

Product Details

16206-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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