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          • Primary Antibodies ›
          • MLKL Antibodies

          Invitrogen

          MLKL Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          5 References
          View all (32) MLKL antibodies

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          Cite MLKL Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Published Figures (4)
          • Advanced Verification (1)
          MLKL Antibody in Western Blot (WB)
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          MLKL Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          MLKL Antibody (PA5-43960) in WB

          Western blot analysis was performed on membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), SW480 (Lane 2), U-937 (Lane 3), HT-29 (Lane 4), Mouse Brain (Lane 5) and Mouse Liver (Lane 6). The blot was probed with Anti-MLKL Polyclonal Antibody (Product # PA5-43960, 1 µg/mL dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 55 kDa band corresponding to MLKL was observed across all the cell lines and ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          MLKL Antibody in Western Blot (WB)
          MLKL Antibody in Western Blot (WB)
          MLKL Antibody in Western Blot (WB)
          MLKL Antibody in Western Blot (WB)
          MLKL Antibody in Western Blot (WB)
          MLKL Antibody in Western Blot (WB)
          MLKL Antibody
          MLKL Polyclonal Antibody

          Product Details

          PA5-43960

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1 µg/mL
          View 5 publications 5 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide directed towards the N-terminal of human MLKL (aa 107-156).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          50 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS with 2% sucrose

          Contains

          0.09% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2606606

          Product Specific Information

          Peptide sequence: DVWKELSLLL QVEQRMPVSP ISQGASWAQE DQQDADEDRR AFQMLRRDNE

          Sequence homology: Cow: 85%; Horse: 91%; Human: 100%; Rat: 90%

          Target Information

          The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: hMLKL; Mixed lineage kinase domain-like protein; unnamed protein product

          View more View less

          Gene Aliases: hMLKL; MLKL

          View more View less

          UniProt ID: (Human) Q8NB16

          View more View less

          Entrez Gene ID: (Human) 197259

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding protein kinase binding identical protein binding macromolecular complex binding
          Process(es)
          cell surface receptor signaling pathway programmed cell death defense response to virus protein homotrimerization necroptotic process necroptotic signaling pathway execution phase of necroptosis
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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