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Invitrogen
IL-4 Monoclonal Antibody (1D11)
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FIGURE: 1 / 1
IL-4 Antibody (MM450C) in ELISA
Sandwich ELISA analysis of mouse IL-4 was performed using a Mouse IL-4 Colorimetric ELISA kit (Product # EMIL4) by loading 50 µL per well of Mouse IL-4 Recombinant Protein (Product # SMIL4) in dodecuplicate at 375, 75, 15 and 0 pg/mL across a 2 µg/mL rat anti-mouse IL-4 (Product # MM450C) pre-coated plate and incubating for 2 hours at 37°C. The plate was washed, then incubated with 100 µL per well of an HRP-conjugated rat anti-mouse IL-4 antibody at a concentration of 0.4 µg/mL for 1 hour at 37°C. Detection was performed using 1-Step Ultra... View More
Product Details
MM450C
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse,
Rat
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
1D11
Immunogen
Recombinant mouse IL-4
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
500 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MM450C targets IL-4 in ELISA, and WB applications and shows reactivity with mouse samples. Does not bind to human IL-4.
The MM450C immunogen is recombinant mouse IL-4.
MM450C detects IL-4 which has a predicted molecular weight of approximately 14 kDa.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
The MM450C mouse IL4 antibody (clone 1D11) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM450DB (biotinylated conjugate of clone 24G2). Typical dilutions for sandwich ELISA include 1-5 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody MM450C (clone 1D11) and biotinylated antibody MM450DB (clone 24G2) have successfully been used in combination with recombinant mouse IL4 protein SMIL4 in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402
Gene Aliases: BSF-1; Il-4; Il4
UniProt ID: (Mouse) P07750
Entrez Gene ID: (Mouse) 16189
microglial cell activation
positive regulation of protein phosphorylation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
positive regulation of immunoglobulin production
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
immune response
cholesterol metabolic process
positive regulation of cell proliferation
regulation of proton transport
negative regulation of epithelial cell migration
positive regulation of B cell proliferation
B cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
T-helper 2 cell cytokine production
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
B cell activation
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
negative regulation of macrophage activation
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
positive regulation of chemokine biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
regulation of immune response
negative regulation of T cell activation
positive regulation of B cell activation
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of myoblast fusion
negative regulation of complement-dependent cytotoxicity
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
negative regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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