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Invitrogen
MMP10 Polyclonal Antibody
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FIGURE: 1 / 6
MMP10 Antibody (PA5-79677) in ICC/IF
Immunocytochemistry analysis of MMP10 using anti-MMP10 antibody (Product # PA5-79677) . MMP10 was detected in a section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MMP10 antibody (Product # PA5-79677) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. is... View More
Product Details
PA5-79677
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human MMP10 recombinant protein (Position: Y239-C476).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat cardiac muscle tissue, mouse cardiac muscle tissue, human placenta tissue. IHC: human colon cancer tissue, human mammary cancer tissue. ICC/IF: A549 cell.
Target Information
MMP10 belongs to the matrix metalloproteinase family, and is generally secreted in its pro form, which is further modified by extracellular proteinases. As a metalloproteinase, MMP10 requires zinc and calcium as cofactors for activity. It is involved in the activation of pro-collagenase, catabolism of collagen, degradation of fibronectin, and tissue remodeling. In humans, the gene MMP10 located on chromosome 11.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: matrix metallo protease; matrix metalloprotease 10; matrix metalloproteinase 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP; MMP-10; MMPs; SL-2; stromelysin 2; Stromelysin-2; Transformation-associated protein 34A; transin 2; Transin-2
Gene Aliases: AV377895; MMP-10; MMP10; SL-2; STMY2
UniProt ID: (Human) P09238, (Mouse) O55123, (Rat) P07152
Entrez Gene ID: (Human) 4319, (Mouse) 17384, (Rat) 117061
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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