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Invitrogen
MMP12 Polyclonal Antibody
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FIGURE: 1 / 2
MMP12 Antibody (PA5-80818) in ICC/IF
Immunofluorescence staining of MMP12 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with MMP12 Polyclonal Antibody (Product # PA5-80818, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-80818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human MMP-12/MMP12 Protein (Gly106-Asn268)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human MMP-12/MMP12.
Target Information
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.4.24.65; Macrophage elastase; Macrophage metalloelastase; matrix metallo protease; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP; MMP-12; MMPs; unnamed protein product
Gene Aliases: HME; ME; MME; MMP-12; MMP12
UniProt ID: (Human) P39900
Entrez Gene ID: (Human) 4321
negative regulation of transcription from RNA polymerase II promoter
proteolysis
protein import into nucleus
positive regulation of gene expression
extracellular matrix disassembly
extracellular matrix organization
collagen catabolic process
positive regulation of interferon-alpha production
wound healing, spreading of epidermal cells
positive regulation of transcription from RNA polymerase II promoter
lung alveolus development
regulation of defense response to virus by host
positive regulation of epithelial cell proliferation involved in wound healing
elastin catabolic process
negative regulation of type I interferon-mediated signaling pathway
positive regulation of type I interferon-mediated signaling pathway
bronchiole development
cellular response to virus
response to amyloid-beta
negative regulation of endothelial cell-matrix adhesion via fibronectin
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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