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MMP13 Polyclonal Antibody

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MMP13 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MMP13 antibody (Product # 18165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MMP13 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 18165-1-AP (MMP13 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MMP13 Antibody in Western Blot (WB) — MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18165-1-AP (MMP13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

18165-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 19 publications 19 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 14 publications 14 publications
Miscellaneous PubMed (Misc)	-	View 4 publications 4 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rabbit, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MMP13 Fusion Protein Ag12653 (100-471 aa encoded by BC074808) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: DVGEYNVFP RTLKWSKMNL TYRIVNYTPD MTHSEVEKAF KKAFKVWSDV TPLNFTRLHD GIADIMISFG IKEHGDFYPF DGPSGLLAHA FPPGPNYGGD AHFDDDETWT SSSKGYNLFL VAAHEFGHSL GLDHSKDPGA LMFPIYTYTG KSHFMLPDDD VQGIQSLYGP GDEDPNPKHP KTPDKCDPSL SLDAITSLRG ETMIFKDRFF WRLHPQQVDA ELFLTKSFWP ELPNRIDAAY EHPSHDLIFI FRGRKFWALN GYDILEGYPK KISELGLPKE VKKISAAVHF EDTGKTLLFS GNQVWRYDDT NHIMDKDYPR LIEEDFPGIG DKVDAVYEKN GYIYFFNGPI QFEYSIWSNR IVRVMPANSI LWC (100-471 aa encoded by BC074808)

Target Information

MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Collagenase; Collagenase 3; collagenase-1; Collagenase-3; Collagenase3; interstitial collagenase; matrix metallo protease; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3; UMRCASE

Gene Aliases: Clg; CLG3; MANDP1; MMP-13; Mmp1; MMP13

UniProt ID: (Human) P45452, (Mouse) P33435

Entrez Gene ID: (Human) 4322, (Mouse) 17386, (Rat) 171052

Molecular Function: metalloprotease

Function(s)

luteolysis osteoblast differentiation response to hypoxia endochondral ossification growth plate cartilage development proteolysis heart development parturition response to mechanical stimulus extracellular matrix disassembly bone mineralization collagen catabolic process embryonic hindlimb morphogenesis response to drug peptide catabolic process response to estrogen cellular protein metabolic process estrous cycle bone morphogenesis cellular response to fluid shear stress positive regulation of pancreatic trypsinogen secretion receptor internalization cartilage development ossification

Process(es)

fibronectin binding metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding collagen binding zinc ion binding calcium-dependent protein binding low-density lipoprotein particle receptor binding peptidase activity metallopeptidase activity hydrolase activity metal ion binding

MMP13 Polyclonal Antibody

Product Details

18165-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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