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          • Primary Antibodies ›
          • MMP16 Antibodies

          Invitrogen

          MMP16 Polyclonal Antibody

          2 Published Figures
          2 References
          View all (13) MMP16 antibodies

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          Cite MMP16 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (7)
          • Published Figures (2)
          MMP16 Antibody in Immunocytochemistry (ICC/IF)
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          MMP16 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          MMP16 Antibody (PA5-79680) in ICC/IF

          Immunocytochemistry analysis of MMP16 using anti-MMP16 antibody (Product # PA5-79680). MMP16 was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MMP16 antibody (Product # PA5-79680) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Vi... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          MMP16 Antibody in Immunocytochemistry (ICC/IF)
          MMP16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MMP16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MMP16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MMP16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          MMP16 Antibody in Western Blot (WB)
          MMP16 Antibody in Flow Cytometry (Flow)
          MMP16 Antibody in Immunohistochemistry (IHC)
          MMP16 Antibody in Immunohistochemistry (IHC)
          MMP16 Polyclonal Antibody

          Product Details

          PA5-79680

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          2 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          0.1-0.5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E. coli-derived human MMP16 recombinant protein (Position: Y120-I296).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 5mg BSA

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2746795

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: mouse intestine tissue, human SMMC-7721 whole cell. IHC: human colon cancer tissue, human lung cancer tissue, human placenta tissue, human mammary cancer tissue. ICC/IF: A431 cell. Flow: CACO-2 cell.

          Target Information

          Matrix metallopeptidase 16 during embryonic development, reproduction, and tissue remodeling. MMP-16 is also involved in the ECM degradation processes associated with arthritis and metastasis. In humans, MMP-16 is a part of a cluster of MMP genes at the 11q22.3 locus.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DKFZp761D112; matrix metallo protease; matrix metallopeptidase 16 (membrane-inserted); matrix metalloproteinase 16; Matrix metalloproteinase-16; Membrane type 3-MMP; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP; MMP-1; MMP-16; MMP-X2; MMPs; MT-MMP 3; MT3-MMP; MT3MMP; MTMMP3; Putative transmembrane protein C8orf57; unnamed protein product

          View more View less

          Gene Aliases: C8orf57; MMP-X2; MMP16; MMPX2; MT-MMP2; MT-MMP3; MT3-MMP; Mt3mmp

          View more View less

          UniProt ID: (Human) P51512, (Rat) O35548, (Mouse) Q9WTR0

          View more View less

          Entrez Gene ID: (Human) 4325, (Rat) 65205, (Mouse) 17389

          View more View less

          Function(s)
          metalloendopeptidase activity enzyme activator activity peptidase activity metallopeptidase activity zinc ion binding hydrolase activity metal ion binding metalloaminopeptidase activity calcium ion binding metalloprotease
          Process(es)
          skeletal system development ossification endochondral ossification proteolysis protein processing extracellular matrix organization collagen catabolic process chondrocyte proliferation embryonic cranial skeleton morphogenesis bone development craniofacial suture morphogenesis
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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