|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues 475-490 of human MMP-2: MGPLLVATFWPELPEK|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:1000|
|Immunohistochemistry (Frozen) (IHC (F))||2-4 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||1:1500-1:3500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form).
Antigen retrieval is not required for IHC.
Suggested positive control: Injured rat nerve lysate.
Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, the signalling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.