|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||E. coli expressed active rat 72-kDa type IV collagenase (catalytic domain).|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22527 detects MMP-2 in Rat samples.
PA1-22527 has been successfully used in Immunoprecipitation and Western Blot procedures.
PA1-22527 immunogen corresponds to Recombinant fragment (catalytic domain) (Rat)..
The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes (ref 1). After cleavage of a signal peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP (ref 2). MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contain the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.