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Invitrogen
MMP2 Monoclonal Antibody (2C1)
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FIGURE: 1 / 4
MMP2 Antibody (MA1-16640) in IHC (P)
Immunohistochemistry analysis of MMP2 in FFPE human breast cancer section. Samples were incubated with MMP2 monoclonal antibody (Product # MA1-16640) using a dilution of 5 µg/mL. The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minu... View More
Product Details
MA1-16640
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Guinea pig,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2C1
Immunogen
Activated recombinant human MMP2.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is not recommended for Immunohistochemistry on paraffin-embedded sections.
Suggested positive control: lung, nerve, and various tumors.
Target Information
MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1
Gene Aliases: CLG4; CLG4A; GelA; MMP-2; MMP-II; MMP2; MONA; TBE-1
UniProt ID: (Human) P08253, (Rat) P33436, (Mouse) P33434
Entrez Gene ID: (Human) 4313, (Guinea pig) 100719361, (Rat) 81686, (Mouse) 17390
angiogenesis
response to hypoxia
blood vessel maturation
intramembranous ossification
proteolysis
embryo implantation
extracellular matrix disassembly
collagen catabolic process
endodermal cell differentiation
cellular protein metabolic process
positive regulation of innate immune response
ephrin receptor signaling pathway
face morphogenesis
bone trabecula formation
cellular response to amino acid stimulus
positive regulation of vascular smooth muscle cell proliferation
ovarian follicle development
ovulation from ovarian follicle
luteinization
response to oxidative stress
negative regulation of cell adhesion
female pregnancy
parturition
aging
response to radiation
response to mechanical stimulus
peripheral nervous system axon regeneration
response to activity
cell migration
positive regulation of cell migration
response to retinoic acid
response to cytokine
response to nicotine
response to drug
response to hydrogen peroxide
positive regulation of apoptotic process
response to estrogen
negative regulation of vasoconstriction
skeletal system morphogenesis
tissue remodeling
response to electrical stimulus
response to hyperoxia
prostate gland epithelium morphogenesis
cellular response to cytokine stimulus
cellular response to interleukin-1
cellular response to estradiol stimulus
cellular response to fluid shear stress
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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