Invitrogen

MMP9 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue using a MMP9 polyclonal antibody (Product # PA5-13199), followed by HRP-conjugated secondary antibody and DAB staining.

MMP9 Antibody in Western Blot (WB) — Western blot analysis using a MMP9 polyclonal antibody (Product # PA5-13199) in mouse spleen lysates (35 µg per lane).

Product Details

PA5-13199

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Ammonium sulfate precipitation, Size-exclusion - Dialysis

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2144757

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRIM66 Promotes Malignant Progression of Non-Small-Cell Lung Cancer Cells via Targeting MMP9.

Computational and mathematical methods in medicine

Xu Y,Yang Q,Fang Z,Tan X,Zhang M,Chen W

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Tue Aug 02 00:00:00 EDT 2022

Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy.

Andrologia

Feng C,Wang Q,Deng L,Peng N,Yang M,Wang X

PA5-13199 was used in Western Blotting to demonstrate that high circ_0074032 level in PCa is associated with worse PCa-related prognosis. Circ_0074032 induced cell apoptosis, curbed cell proliferation, migration and invasion in PCa cells and was identified as miR-198 decoy. Circ_0074032 regulates HOXA1 expression and promotes PCa progression via elevating HOXA1 expression through acting as a miR-198 sponge.

Tue Feb 01 00:00:00 EST 2022

Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.

Journal of orthopaedic surgery and research

Wang T,Zhang C,Wang S

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Mon Dec 20 00:00:00 EST 2021

Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.

Pharmaceuticals (Basel, Switzerland)

Gajda-Morszewski P,Gurgul I,Janczy-Cempa E,Mazuryk O,Łomzik M,Brindell M

PA5-13199 was used in Western Blotting to show a reduction of metastatic cells escaping from the primary lesion to the surrounding tissue by prevention of their detachment and by influencing the activity of MMP-2 and MMP-9.

Fri Oct 01 00:00:00 EDT 2021

Inhibition of Matrix Metalloproteinases and Cancer Cell Detachment by Ru(II) Polypyridyl Complexes Containing 4,7-Diphenyl-1,10-phenanthroline Ligands-New Candidates for Antimetastatic Agents.

Pharmaceuticals (Basel, Switzerland)

Gajda-Morszewski P,Gurgul I,Janczy-Cempa E,Mazuryk O,Łomzik M,Brindell M

Fri Oct 01 00:00:00 EDT 2021

Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.

Pharmaceuticals (Basel, Switzerland)

Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T

PA5-13199 was used in Western Blot to test the hypothesis that endothelial-specific thioredoxin-interacting protein knock-out mice will be more resistant to the neurovascular damage associated with hyperglycemia in embolic stroke.

Mon Sep 27 00:00:00 EDT 2021

Endothelial Thioredoxin-Interacting Protein Depletion Reduces Hemorrhagic Transformation in Hyperglycemic Mice after Embolic Stroke and Thrombolytic Therapy.

Pharmaceuticals (Basel, Switzerland)

Salman M,Ismael S,Li L,Ahmed HA,Puchowicz MA,Ishrat T

Mon Sep 27 00:00:00 EDT 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

PA5-13199 was used in Western Blotting to show that KRL not only activates the IGF-IR-dependent p-PI3K/p-AKT and Nrf2 signaling pathway, but also suppresses caspase-dependent apoptosis.

Tue Mar 23 00:00:00 EDT 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

PA5-13199 was used in Western Blotting to show that KRL not only activates the IGF-IR-dependent p-PI3K/p-AKT and Nrf2 signaling pathway, but also suppresses caspase-dependent apoptosis.

Tue Mar 23 00:00:00 EDT 2021

Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.

Journal of chemotherapy (Florence, Italy)

Li SL,Jiang TQ,Cao QW,Liu SM

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Mon Feb 01 00:00:00 EST 2021

CCL4 Inhibition in Atherosclerosis: Effects on Plaque Stability, Endothelial Cell Adhesiveness, and Macrophages Activation.

International journal of molecular sciences

Chang TT,Yang HY,Chen C,Chen JW

PA5-13199 was used in Immunohistochemistry-immunofluorescence to investigate whether CCL4 inhibition could retard the progression of atherosclerosis.

Tue Sep 08 00:00:00 EDT 2020

EphrinA1-Fc Attenuates Ventricular Remodeling and Dysfunction in Chronically Nonreperfused WT but not EphA2-R-M mice.

International journal of molecular sciences

Whitehurst KS,Chan VA,Estes HK,Valsaraj S,Kent S,Sharma UM,Chase RC,Bhuiyan M,Virag JAI

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Thu Aug 13 00:00:00 EDT 2020

Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears.

Folia morphologica

Atiç R,Deveci E

PA5-13199 was used in Immunohistochemistry to study the effects of meniscal tears in inflamed joints in terms of cytokine release.

Tue Jan 21 00:00:00 EST 2020

Expression of matrix metalloproteinase-9 and tumour necrosis factor-alpha in the synovial cells of patients with meniscus tears.

Folia morphologica

Atiç R,Deveci E

PA5-13199 was used in Immunohistochemistry to study the effects of meniscal tears in inflamed joints in terms of cytokine release.

Tue Jan 21 00:00:00 EST 2020

Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Deng Z,Gao X,Sun X,Amra S,Lu A,Cui Y,Eltzschig HK,Lei G,Huard J

PA5-13199 was used in Immunohistochemistry-immunofluorescence to investigate articular cartilage (AC) homeostasis and signaling pathways involved in the superior cartilage regeneration of Murphy Roths large (MRL/MpJ) mice.

Thu Aug 01 00:00:00 EDT 2019

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

Cell biology international

Zhang Y,Zhang Y

Published figure using MMP9 polyclonal antibody (Product # PA5-13199) in Western Blot

Sun Jul 01 00:00:00 EDT 2018

Intranasal Curcumin Inhibits Pulmonary Fibrosis by Modulating Matrix Metalloproteinase-9 (MMP-9) in Ovalbumin-Induced Chronic Asthma.

Inflammation

Chauhan PS,Dash D,Singh R

PA513199 was used in western blot to study the effect of intranasal curcumin on airway remodeling and fibrosis in murine model of chronic asthma

Wed Feb 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; collagenase type IVB; Gel B; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

Gene Aliases: AW743869; B/MMP9; CLG4B; GELB; MANDP2; MMP-9; MMP9; pro-MMP-9

UniProt ID: (Human) P14780, (Mouse) P41245

Entrez Gene ID: (Human) 4318, (Mouse) 17395

Molecular Function: metalloprotease