Invitrogen

MMP9 Monoclonal Antibody (5G3)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

13 Published Figures

16 References

View all (52) MMP-9 antibodies

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MMP9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Matrix metalloproteinase-9 was performed using 70% confluent log phase THP-1 cells differentiated to Macrophages using PMA (100nM 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MMP9 Monoclonal Antibody (5G3) (Product # MA5-15886) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasmic localization. Panel e represents undifferentiated THP-1 cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded brain tissues using MMP9 monoclonal antibody (Product # MA5-15886) followed with DAB staining.

MMP9 Antibody in Western Blot (WB) — Western blot was performed using Anti-MMP9 Monoclonal Antibody (5G3) (Product # MA5-15886) and a ~92 kDa (Pro-form) band corresponding to Matrix metalloproteinase-9 was observed THP-1 cells. Also along with it, an ~82 kDa band corresponding to active form of MMP9 was observed upon treatment with PMA (100 nM, 48 hours;) and also further upregulated upon treatment with both PMA (100 nM, 48 hours) and Protein Transport Inhibitor (PTI) (1X, 4 hours). Whole cell extracts (40 µg lysate) of THP-1 (Lane 1), THP-1 treated with PTI (Lane 2), THP-1 treated with PMA (Lane 3) and THP-1 treated with PMA and PTI (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

MMP9 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HeLa cells using MMP9 monoclonal antibody (Product # MA5-15886) (blue) and negative control (red).

MMP9 Antibody in Immunohistochemistry (IHC) — FIGURE 8 Inhibition of circ_0058357 represses tumor growth and sensitizes A549/DDP cells to DDP in vivo. (a)-(e) A549/DDP cells infected with sh-circ_0058357 or sh-NC were injected (4 x 10 6 cells each mouse) subcutaneously into BALB/c nude mice. After eight days, DDP (30 mg/kg, every 3 days) or PBS administration and tumor volume measurement began. Each group included six mice. Twenty-three days later, tumors were collected. (a) Growth curve of the tumors. (b) Representative images and mean weight of the xenograft tumors. (c) qRT-PCR analysis of circ_0058357 and miR-361-3p levels in the tumors. (d) Western blot showing ABCC1 protein level in the excised tumors. (e) Immunohistochemistry of ABCC1 and MMP9 protein levels in the tumors. Scale bar: 100 mum. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

MMP9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with Matrix metalloproteinase-9 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-MMP9 Monoclonal Antibody (5G3) (Product # MA5-15886). {KD}

Product Details

MA5-15886

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 13 publications 13 publications
Immunohistochemistry (IHC)	1:200-1:1,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:200-1:1,000	View 2 publications 2 publications
Flow Cytometry (Flow)	1/200 - 1/400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	5G3
Immunogen	Purified recombinant fragment of human MMP9 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11157246

Product Specific Information

MA5-15886 targets MMP9 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15886 immunogen is purified recombinant fragment of human MMP9 expressed in E. Coli. .

MA5-15886 detects MMP9 which has a predicted molecular weight of approximately 92kDa.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EIF5A2 Is Involved in the Biological Process of Cervical Cancer Cells through AGR2.

Pharmacology

Shen X,Li L,He Y,Lv X,Ma J

MA5-15886 was used in Western Blotting to show that EIF5A2 knockdown inhibited the biological process of cervical cancer cells through modulation of AGR2.

Wed Jul 06 00:00:00 EDT 2022

TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways.

Cell death & disease

Wang LN,Zhang ZT,Wang L,Wei HX,Zhang T,Zhang LM,Lin H,Zhang H,Wang SQ

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Thu May 19 00:00:00 EDT 2022

Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line.

Revista da Associacao Medica Brasileira (1992)

Karagulle OO,Yurttas AG

MA5-15886 was used in Immunocytochemistry to examine the potential anticancer effects of ozone applied after chemotherapeutic treatment with different concentrations of doxorubicin in Luminal-A subtype of human breast cancer cell line (MCF-7) and compare the results with effects on L929 fibroblast cell line.

Fri Apr 01 00:00:00 EDT 2022

Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578.

Cell death discovery

Yan P,Sun C,Luan L,Han J,Qu Y,Zhou C,Xu D

MA5-15886 was used in Western Blot to suggest that circ_0134111 could promote the progression of IDD through enhancing aberrant NP cell growth, inflammation, and ECM degradation partly via regulating miR-578.

Tue Feb 08 00:00:00 EST 2022

Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578.

Cell death discovery

Yan P,Sun C,Luan L,Han J,Qu Y,Zhou C,Xu D

Tue Feb 08 00:00:00 EST 2022

Identification of circ_0058357 as a regulator in non-small cell lung cancer cells resistant to cisplatin by miR-361-3p/ABCC1 axis.

Thoracic cancer

Chu D,Li P,Li Y,Shi J,Huang S,Jiao P

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Immunohistochemistry

Mon Nov 01 00:00:00 EDT 2021

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Biomolecules

Juin SK,Pushpakumar S,Sen U

MA5-15886 was used in Western Blot to suggest that RXR signaling plays a significant role in ECM turnover, and GYY4137 modulates PPAR/RAR-mediated RXR signaling to ameliorate PAI-1-dependent adverse ECM turnover in DN.

Thu Oct 07 00:00:00 EDT 2021

GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.

Biomolecules

Juin SK,Pushpakumar S,Sen U

Thu Oct 07 00:00:00 EDT 2021

Adiponectin and Leptin Exert Antagonizing Effects on HUVEC Tube Formation and Migration Modulating the Expression of CXCL1, VEGF, MMP-2 and MMP-9.

International journal of molecular sciences

Nigro E,Mallardo M,Polito R,Scialò F,Bianco A,Daniele A

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Tue Jul 13 00:00:00 EDT 2021

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

MA5-15886 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 EDT 2021

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

MA5-15886 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 EDT 2021

Exogenous hydrogen sulfide and miR-21 antagonism attenuates macrophage-mediated inflammation in ischemia reperfusion injury of the aged kidney.

GeroScience

Pushpakumar S,Kundu S,Weber G,Sen U

MA5-15886 was used in Western Blot to determine the effects of age-induced reduction in hydrogen sulfide and mir-21 antagonism in acute kidney injury.

Tue Jun 01 00:00:00 EDT 2021

MUC 15 Promotes Osteosarcoma Cell Proliferation, Migration and Invasion through Livin, MMP-2/MMP-9 and Wnt/β-Catenin Signal Pathway.

Journal of Cancer

Chen T,Chen Z,Lian X,Wu W,Chu L,Zhang S,Wang L

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Tue Jan 05 00:00:00 EST 2021

Sevoflurane inhibits the proliferation and invasion of hepatocellular carcinoma cells through regulating the PTEN/Akt/GSK‑3β/β‑catenin signaling pathway by downregulating miR‑25‑3p.

International journal of molecular medicine

Cao Y,Lv W,Ding W,Li J

MA5-15886 was used in Western Blotting to determine the molecular mechanisms governing sevoflurane's action in hepatocellular carcinoma cells.

Wed Jul 01 00:00:00 EDT 2020

Sevoflurane inhibits the proliferation and invasion of hepatocellular carcinoma cells through regulating the PTEN/Akt/GSK‑3β/β‑catenin signaling pathway by downregulating miR‑25‑3p.

International journal of molecular medicine

Cao Y,Lv W,Ding W,Li J

MA5-15886 was used in Western Blotting to determine the molecular mechanisms governing sevoflurane's action in hepatocellular carcinoma cells.

Wed Jul 01 00:00:00 EDT 2020

miR-939-3p promotes epithelial-mesenchymal transition and may be used as a prognostic marker in hepatocellular carcinoma.

Oncology letters

Chen F,Ni X,Chen L,Wang X,Xu J

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Wed Apr 01 00:00:00 EDT 2020

Hydrogen sulphide mitigates homocysteine-induced apoptosis and matrix remodelling in mesangial cells through Akt/FOXO1 signalling cascade.

Cellular signalling

Majumder S,Ren L,Pushpakumar S,Sen U

MA5-15886 was used in Western Blot to conclude that mesangial cell damage and extracellular remodelling caused by hyperhomocysteinemia is regulated by the Akt/FOXO1 pathway.

Sun Sep 01 00:00:00 EDT 2019

MMP-2 and MMP-9 as prognostic markers for the early detection of urinary bladder cancer.

Journal of biochemical and molecular toxicology

Fouad H,Salem H,Ellakwa DE,Abdel-Hamid M

Published figure using MMP9 monoclonal antibody (Product # MA5-15886) in Western Blot

Mon Apr 01 00:00:00 EDT 2019

Characterization of metastatic tumor antigen 1 and its interaction with hepatitis B virus X protein in NF-κB signaling and tumor progression in a woodchuck hepatocellular carcinoma model.

Oncotarget

Li YT,Liu CJ,Su TH,Cheng HR,Jeng YM,Lin HL,Wang CC,Kao JH,Chen PJ,Chen DS,Wu HL

MA515886 was used in western blot to explore the role of metastatic tumor antigen 1 in hepatitis B-associated hepatocarcinogenesis in the woodchuck model

Tue Jul 26 00:00:00 EDT 2016

An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells.

Journal of leukocyte biology

Naegelen I,Plançon S,Nicot N,Kaoma T,Muller A,Vallar L,Tschirhart EJ,Bréchard S

MA5-15886 was used in immunocytochemistry to elucidate the intracellular SNARE machinery responsible for the regulation of cytokine secretion and degranulation.

Sun Mar 01 00:00:00 EST 2015

Bioinformatics

Protein Aliases: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; 92kD gelatinase; 92kD type IV collagenase; 92kDa gelatinase; 92kDa type IV collagenase; collagenase type IVB; Gel B; Gelatinase B; Gelatinase-B; GELB; macrophage gelatinase; matrix metallo protease; matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); Matrix metalloproteinase-9; Matrix metalloproteinase9; MMP; MMP-9; MMPs; type V collagenase

Gene Aliases: AW743869; B/MMP9; CLG4B; GELB; MANDP2; MMP-9; MMP9; pro-MMP-9

UniProt ID: (Human) P14780, (Mouse) P41245

Entrez Gene ID: (Human) 4318, (Mouse) 17395

Molecular Function: metalloprotease

Function(s)

Process(es)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity protein binding collagen binding metallopeptidase activity zinc ion binding identical protein binding fibronectin binding peptidase activity hydrolase activity protein complex binding metal ion binding

MMP9 Monoclonal Antibody (5G3)

Product Details

MA5-15886

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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