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MMS19 Polyclonal Antibody

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Antibody Testing Data (9)

MMS19 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

MMS19 Antibody (16015-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 16015-1-AP (MMS19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

16015-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MMS19 Fusion Protein Ag8792 (457-803 aa encoded by BC006575)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FLDGNVSFL PENSFPSRFQ PFQDGSSGQR RLIALLMAFV CSLPRNVEIP QLNQLMRELL ELSCCHSCPF SSTAAAKCFA GLLNKHPAGQ QLDEFLQLAV DKVEAGLDSG PCRSQAFTLL LWVTKALVLR YHPLSSCLTA RLMGLLSDPE LGPAAADGFS LLMSDCTDVL TRAGHAEVRI MFRQRFFTDN VPALVQGFHA APQDVKPNYL KGLSHVLNRL PKPVLLPELP TLLSLLLEAL SCPDCVVQLS TLSCLQPLLL EAPQVMSLHV DTLVTKFLNL SSSPSMAVRI AALQCMHALT RLPTPVLLPY KPQVIRALAK PLDDKKRLVR KEAVSARGEW FLLGSPGS (457-803 aa encoded by BC006575)

Target Information

Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription. As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Iron-regulated assembly of the cytosolic iron-sulfur cluster biogenesis machinery.

The Journal of biological chemistry

Fan X,Barshop WD,Vashisht AA,Pandey V,Leal S,Rayatpisheh S,Jami-Alahmadi Y,Sha J,Wohlschlegel JA

Fri Jul 01 00:00:00 EDT 2022

Structural basis for substrate recognition and chemical inhibition of oncogenic MAGE ubiquitin ligases.

Nature communications

Yang SW,Huang X,Lin W,Min J,Miller DJ,Mayasundari A,Rodrigues P,Griffith EC,Gee CT,Li L,Li W,Lee RE,Rankovic Z,Chen T,Potts PR

Thu Oct 01 00:00:00 EDT 2020

The iron-sulfur helicase DDX11 promotes the generation of single-stranded DNA for CHK1 activation.

Life science alliance

Simon AK,Kummer S,Wild S,Lezaja A,Teloni F,Jozwiakowski SK,Altmeyer M,Gari K

Sun Mar 01 00:00:00 EST 2020

The iron-sulphur cluster in human DNA2 is required for all biochemical activities of DNA2.

Communications biology

Mariotti L,Wild S,Brunoldi G,Piceni A,Ceppi I,Kummer S,Lutz RE,Cejka P,Gari K

Tue Jun 23 00:00:00 EDT 2020

Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.

Haematologica

Maio N,Kim KS,Holmes-Hampton G,Singh A,Rouault TA

Sun Sep 01 00:00:00 EDT 2019

Sideroflexin 4 affects Fe-S cluster biogenesis, iron metabolism, mitochondrial respiration and heme biosynthetic enzymes.

Scientific reports

Paul BT,Tesfay L,Winkler CR,Torti FM,Torti SV

Mon Dec 23 00:00:00 EST 2019

Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.

Cell

Ast T,Meisel JD,Patra S,Wang H,Grange RMH,Kim SH,Calvo SE,Orefice LL,Nagashima F,Ichinose F,Zapol WM,Ruvkun G,Barondeau DP,Mootha VK

Thu May 30 00:00:00 EDT 2019

Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.

Molecular cell

Weon JL,Yang SW,Potts PR

Thu Jan 04 00:00:00 EST 2018

Cytosolic Iron-Sulfur Assembly Is Evolutionarily Tuned by a Cancer-Amplified Ubiquitin Ligase.

Molecular cell

Weon JL,Yang SW,Potts PR

Thu Jan 04 00:00:00 EST 2018

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Human molecular genetics

Kim KS,Maio N,Singh A,Rouault TA

Thu Mar 01 00:00:00 EST 2018

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.

Human molecular genetics

Kim KS,Maio N,Singh A,Rouault TA

Thu Mar 01 00:00:00 EST 2018

The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.

Cell reports

Odermatt DC,Gari K

Tue Feb 07 00:00:00 EST 2017

The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.

Cell reports

Odermatt DC,Gari K

Tue Feb 07 00:00:00 EST 2017

Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue.

Molecular metabolism

Palmer CJ,Bruckner RJ,Paulo JA,Kazak L,Long JZ,Mina AI,Deng Z,LeClair KB,Hall JA,Hong S,Zushin PH,Smith KL,Gygi SP,Hagen S,Cohen DE,Banks AS

Sun Oct 01 00:00:00 EDT 2017

Methyl-methanesulfonate sensitivity 19 expression is associated with metastasis and chemoradiotherapy response in esophageal cancer.

World journal of gastroenterology

Zhang JL,Wang HY,Yang Q,Lin SY,Luo GY,Zhang R,Xu GL

Tue Apr 14 00:00:00 EDT 2015

Methyl-methanesulfonate sensitivity 19 expression is associated with metastasis and chemoradiotherapy response in esophageal cancer.

World journal of gastroenterology

Zhang JL,Wang HY,Yang Q,Lin SY,Luo GY,Zhang R,Xu GL

Tue Apr 14 00:00:00 EDT 2015

The Association of the Xeroderma Pigmentosum Group D DNA Helicase (XPD) with Transcription Factor IIH Is Regulated by the Cytosolic Iron-Sulfur Cluster Assembly Pathway.

The Journal of biological chemistry

Vashisht AA,Yu CC,Sharma T,Ro K,Wohlschlegel JA

Fri May 29 00:00:00 EDT 2015

The Association of the Xeroderma Pigmentosum Group D DNA Helicase (XPD) with Transcription Factor IIH Is Regulated by the Cytosolic Iron-Sulfur Cluster Assembly Pathway.

The Journal of biological chemistry

Vashisht AA,Yu CC,Sharma T,Ro K,Wohlschlegel JA

Fri May 29 00:00:00 EDT 2015

Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins.

Cell metabolism

Stehling O,Mascarenhas J,Vashisht AA,Sheftel AD,Niggemeyer B,Rösser R,Pierik AJ,Wohlschlegel JA,Lill R

Tue Aug 06 00:00:00 EDT 2013

Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins.

Cell metabolism

Stehling O,Mascarenhas J,Vashisht AA,Sheftel AD,Niggemeyer B,Rösser R,Pierik AJ,Wohlschlegel JA,Lill R

Tue Aug 06 00:00:00 EDT 2013

MicroRNA-31 modulates tumour sensitivity to radiation in oesophageal adenocarcinoma.

Journal of molecular medicine (Berlin, Germany)

Lynam-Lennon N,Reynolds JV,Marignol L,Sheils OM,Pidgeon GP,Maher SG

Sat Dec 01 00:00:00 EST 2012

MicroRNA-31 modulates tumour sensitivity to radiation in oesophageal adenocarcinoma.

Journal of molecular medicine (Berlin, Germany)

Lynam-Lennon N,Reynolds JV,Marignol L,Sheils OM,Pidgeon GP,Maher SG

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: hMMS19; homolog of yeast MMS19; incisor protein; MET18 homolog; MMS19 cytosolic iron-sulfur assembly component; MMS19 nucleotide excision repair homolog; MMS19 nucleotide excision repair protein homolog; MMS19 nucleotide excision repair-like; MMS19-like (MET18 homolog, S. cerevisiae); MMS19-like protein

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Gene Aliases: 2410001K24Rik; 2610042O15Rik; AI316855; C79368; C86341; hMMS19; MET18; MMS19; MMS19L

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UniProt ID: (Human) Q96T76, (Mouse) Q9D071

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Entrez Gene ID: (Human) 64210, (Rat) 171124, (Mouse) 72199

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Function(s)

transcription coactivator activity protein binding enzyme binding receptor signaling complex scaffold activity estrogen receptor binding protein binding, bridging scaffold/adaptor protein

Process(es)

phosphorelay signal transduction system DNA metabolic process DNA repair nucleotide-excision repair transcription, DNA-templated cellular response to DNA damage stimulus chromosome segregation response to hormone iron-sulfur cluster assembly positive regulation of transcription, DNA-templated regulation of transcription, DNA-templated

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MMS19 Polyclonal Antibody

MMS19 Antibody (16015-1-AP) in ICC/IF

Product Details

16015-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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